Cell Cytotoxicity Assay Kit II (WST-SRB-Crystal Violet)

• Catalog number
  K173-1000
• Price
  Please ask
• Size
  1000 assays

• Description
  Sensitive, Non-radioactive Assay, measures cytotoxicity using 3 different methods
• Summary
  • Detection- Absorbance [OD: WST/NR/CV: 450 /565/570 nm • Species reactivity- Mammalian • Kit size- 1000 • Applications- The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
• Detection Method
  Absorbance [OD: WST/NR/CV: 450 /565/570 nm
• Species Reactivity
  Eukaryotes
• Applications
  The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
• Sample Type
  Cell cultures (adherent and suspension)
• Features Benefits
  • Simple procedures; take less than 4 hours • Convenient • Uses a water-soluble tetrazolium salt WST reagent, SRB and Crystal Violet . Method are simple and do not require extensive washing/harvesting/or solubilization steps. The entire assay can be performed directly in a 96-well plate.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Cytotoxicity assays are essential tools in drug discovery. In vitro single parameter cytotoxicity assays, such as using tetrazolium salt (WST), sulforhodamine B (SRB) or crystal violet (CV) are widely used in cultured cellsin order to assess the cytotoxicity of potential drug candidates as these methods are fast, reproducible, and do no require using living mammals during early drug discovery stages. However, the most critical problem of using single parameter assays is the determination of their IC50 data offers a limited perspective and can lead to erroneous assumptions or erroneous, unsucessful results when the selected candidates are used in more complex models (false positives). On the other hand, if the cytotoxicity assay is not selected appropriately, false negative compounds that are actually cytotoxic may be easily missed. Thus, the analysis of candidates as potential cytotoxic compounds is usually more comprehensive when two or more cytotoxicity assays are performed. Additionally, SRB, WST and CV cyototoxic assays offer relatively inexpensive, accurate, yet reliable results in a timely manner. BioVision’s WST-SRB-Crystal Violet Combined Cytotoxicity Assay Kit allow researchers to test the cytotoxicity effect of their candidate compounds in different biological processes: cell proliferation (WST), protein synthesis (SRB) and/or total DNA synthesis (CV). The assays are compatible to each other and same set of cultured cells can be tested using these assays. The kit is simple, fast, high-throughput compatible and provides an efficient research tool for the quantitative measurement of cell cytotoxicity in terms of viability, protein and DNA synthesis
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Cytotoxicity   Kit   WST-SRB-Crystal   Violet  
• Short name
  Cytotoxicity Assay Kit II (WST-SRB-Crystal Violet)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  cellular Cytotoxicity test reagent II (WST-SRB-Crystal Violet)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

MeSH Data

• Name
  Crystallization
• Concept
  Scope note: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
• Tree numbers
  • E05.196.300
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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