Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Storage & Transport Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
For quantitative measurement of human IL-7 in cell culture supernatant, serum and plasma EDTA .
More gene info
IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7(IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor(HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta(TCRB) during early T cell development.
ELISA's sensitivity< 1pg/ml
Gen protein identity
Expression system for standard: E.coli; Immunogen sequence: D26-H177
the ELISA does not cross react with any other proteins except from closely related species
ELISA standard curve
Concentration(pg/ml) + 0 + 7.8 + 15.6 + 31.3 + 62.5 + 125 + 250 + 500 O.D. + 0.022 + 0.066 + 0.115 + 0.217 + 0.461 + 0.966 + 1.605 + 2.331
ELISA kit description
GENTAUR's human IL-7 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-7 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, D26-H177) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-7 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL-7 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Minutes of TMB incubation
How the elisa works
Sample the amount of 100ul/well of the standard solution at 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human IL-7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma (EDTA) to the ELISA plate. Keep in mind that each human IL-7 standard solution and each sample be measured in duplicate.
IL 7;IL7 nirs variant 6;Interleukin 7;Interleukin7
There is no detectable cross-reactivity with other relevant proteins.
ELISA kit detection limits
7.8pg/ml to 500pg/ml
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human IL-7 antibodies;1 ST0000-10;Lyophilized recombinant human IL-7 standard;10ng/tube×2 AR1107;Biotinylated anti- human IL-7 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
cell culture supernates, serum and plasma(EDTA).
Target of this ELISA
Natural and recombinant human IL-7
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);44;127;283;67;158;322 ELISA standard deviations 2.33;5.71;13.86;4.15;10.42;17.06 CV(%);5.3;4.5;4.9;6.2;6.6;5.3
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
IL-7 PicoKine ELISA Kit
ELISA kit, ELISA
antigen assay ELISA kit 1 Kit (96 Wells)
H. sapiens Interleukin-7 PicoKine Enzyme-linked immunosorbent assay reagent
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832