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Human IL-7 PicoKine ELISA Kit

Human IL-7 PicoKine ELISA Kit is available 2 times from Boster labs

EK0779 | Human IL-7 PicoKine ELISA Kit size: 96wells/kit, with removable strips. | 568.55 USD

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EK0779 | Human IL-7 PicoKine ELISA Kit size: 1x96 well plate | 604.94 USD

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Keep at Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Storage & Transport Conditions Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
ELISA's application For quantitative measurement of human IL-7 in cell culture supernatant, serum and plasma EDTA .
More gene info IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7(IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor(HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta(TCRB) during early T cell development.
Anti-coagulation EDTA
ELISA's sensitivity< 1pg/ml
Kit Components 10ng/tube
Uniprot Interleukin-7
Gen protein identity P13232
Immunogen Expression system for standard: E.coli; Immunogen sequence: D26-H177
Cross reaction the ELISA does not cross react with any other proteins except from closely related species
Gene tested Human IL-7
ELISA standard curve Concentration(pg/ml) + 0 + 7.8 + 15.6 + 31.3 + 62.5 + 125 + 250 + 500 O.D. + 0.022 + 0.066 + 0.115 + 0.217 + 0.461 + 0.966 + 1.605 + 2.331
Gene name interleukin 7
ELISA kit description GENTAUR's human IL-7 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-7 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, D26-H177) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-7 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL-7 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Minutes of TMB incubation 15-20
How the elisa works Sample the amount of 100ul/well of the standard solution at 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human IL-7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma (EDTA) to the ELISA plate. Keep in mind that each human IL-7 standard solution and each sample be measured in duplicate.
Alternative IL 7;IL7 nirs variant 6;Interleukin 7;Interleukin7
Applications ELISA
Entrez nr. 3574
Cross-reactivity There is no detectable cross-reactivity with other relevant proteins.
ELISA kit detection limits 7.8pg/ml to 500pg/ml
The ELISA contains Catalog number;Description;Quantity -;96-well plate precoated with anti- human IL-7 antibodies;1 ST0000-10;Lyophilized recombinant human IL-7 standard;10ng/tube×2 AR1107;Biotinylated anti- human IL-7 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Antigen E.coli, D26-H177
Reactivity Human
Gene Name IL7
Assay range 7.8pg/ml-500pg/ml
Sample Type cell culture supernates, serum and plasma(EDTA).
Target of this ELISA Natural and recombinant human IL-7
ELISA's precision parameters intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);44;127;283;67;158;322 ELISA standard deviations 2.33;5.71;13.86;4.15;10.42;17.06 CV(%);5.3;4.5;4.9;6.2;6.6;5.3
Detector and capure antibodies a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene targetIL-7 PicoKine
Gene symbol IL7
Short name IL-7 PicoKine ELISA Kit
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Human
Alternative name H. sapiens Interleukin-7 PicoKine Enzyme-linked immunosorbent assay reagent
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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