Human Recombinant Cyclophilin B
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Catalog number6310-100
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PricePlease ask
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Size100 ug
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SynonymsHuman Recombinant Cyclophilin B
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Alternative_namesPPIB, Peptidyl prolyl isomerase B, CYPB; SCYLP; CYP-S1
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DescriptionAn immunophilin family protein catalyzing the isomerization of peptide bonds
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Endotoxin Level< 1.0 EU per 1 ug of protein (determined by LAL method)
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Biological activitySpecific activity is > 220 nmoles/min/mg
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Unit DefinitionSpecific activity is defined as the amount of enzyme that cleaves 1 µmole of Suc-AAFP-pNA per minute at 25°C in Tris HCl pH 8.0 using chymotrypsin.
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Molecular Weight21.2 kDa
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form description1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20 mM NaCl, 0.5 mM DTT, 10% glycerol.
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Background InformationCyclophilin B is a cyclosporine-binding protein. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. It also bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression.
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Amino acid sequenceMLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolPPIB, CAMLG
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Short nameRecombinant Cyclophilin B
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameH. sapiens Rec. Cyclophilin B
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namepeptidylprolyl isomerase B
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-11-25
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Entrez gene record
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Pubmed identfication
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Classification
- Cyclophilin peptidylprolyl isomerases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecalcium modulating ligand
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-02-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Guided entry of tail-anchored proteins complex subunits
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data