MAP2a,b (Microtubule Associated Protein 2a,b (FITC) Antibody
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Catalog number
GEN608400
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Price
Please ask
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Size
0.25 miligrams
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Also known as
MAP2a,b
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Other names
MAP2 protein, partial; Microtubule-associated protein 2; microtubule-associated protein 2; MAP-2; microtubule-associated protein 2; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
MAP2; MAP2; MAP2A; MAP2B; MAP2C; MAP-2
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Gene name synonims
MAP2; MAP2; MAP2A; MAP2B; MAP2C; MAP-2
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Other gene names
MAP2; MAP2; MAP2A; MAP2B; MAP2C; MAP-2
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
1.B.642
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Host organism
Mouse (Mus musculus)
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Source organism
Bovine
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Species reactivity
Bovine, Chicken, Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes (Mr 300kDa) MAP-2 protein from bovine brain. The antibody strongly cross- reacts with MAP-2 from human, rat, mouse and chicken brains. Anti-MAP-2 reacts with the high MW forms (2a & 2b) of MAP-2 but not with the low MW form (2c). Reacts with dendrites and cell bodies of neurons; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Protein G chromatography.. Fluorescein which has a maximum absorbance at 492nm and an emission maximum at 518nm has been covalently attached to Anti-MAP-2 and purified to assure optimal fluorochrome/protein (F/P) molar ratios
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Form Appearance
Purified, Liquid 0.01M PBS, pH 7.2 containing 2.0mM EDTA, 1.0% BSA. 0.1% sodium azide
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Concentration
N/A
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Storage and shipping
Store the antibody at -20 degrees Celsius.
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Tested applications
Immunohistochemistry (IHC)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
MAP2a b (Microtubule Associated Protein 2a b (FITC) Antibody
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Anti-MAP2a,b (Microtubule Associated Protein 2a,b (FITC)
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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