CYR61 Recombinant Protein
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Catalog number
40-221
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Price
Please ask
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Size
0.005 mg, 0.02 mg
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About recombinant protein
CYR61 is a member of the CCN family of secreted cysteine rich regulatory proteins. CYR61 induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells. Cell migration and adhesion are mediated through binding to specific cell surface integrins and to heparin sulfate proteoglycans. Increased expression of CYR61 is associated with several types of cancer, and correlates with the progression and estrogen independence of human breast cancers. Recombinant human CYR61 is a 39.5 kDa protein containing 357 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).
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Other name of the recombinant
CCN1, GIG1, IGFBP10, CCN1, Protein CYR61, CCN family member 1, IBP-10
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Recombinant in
E. coli
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SEQUENCE
MTCPAACHCP LEAPKCAPGV GLVRDGCGCC KVCAKQLNED CSKTQPCDHT KGLECNFGAS STALKGICRA QSEGRPCEYN SRIYQNGESF QPNCKHQCTC IDGAVGCIPL CPQELSLPNL GCPNPRLVKV TGQCCEEWVC DEDSIKDPME DQDGLLGKEL GFDASEVELT RNNELIAVGK GSSLKRLPVF GMEPRILYNP LQGQKCIVQT TSWSQCSKTC GTGISTRVTN DNPECRLVKE TRICEVRPCG QPVYSSLKKG KKCSKTKKSP EPVRFTYAGC LSVKKYRPKY CGSCVDGRCC TPQLTRTVKM RFRCEDGETF SKNVMMIQSC KCNYNCPHAN EAAFPFYRLF NDIHKFRD
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Activity
Biological activity was determined by the dose - dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0 - 3.0 μg/mL.
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Keep at
The lyophilized CYR61 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted CYR61 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing CYR61 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
31542331
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Accession nr
NP_001545.2
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Pureity
Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
3491
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Gene name
cysteine-rich, angiogenic inducer, 61
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Source
Homo sapiens
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Uniprot
O00622
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CYR61
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Short name
CYR61 Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
cysteine-rich, angiogenic inducer, 61 Rec. Protein
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Alternative technique
rec
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Alternative to gene target
cysteine-rich, angiogenic inducer, 61, CCN1 and GIG1 and IGFBP10, CYR61 and IDBG-100054 and ENSG00000142871 and 3491, extracellular matrix binding, Extracellular, Cyr61 and IDBG-198706 and ENSMUSG00000028195 and 16007, CYR61 and IDBG-637282 and ENSBTAG00000009844 and 508941
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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