MIA, human recombinant
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Catalog number4887-1000
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PricePlease ask
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Size1 mg
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SynonymsMelanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA
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Alternative_namesMelanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA, Melanoma inhibitory activity protein
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DescriptionAn inhibitor of in vitro growth of malignant melanoma cells
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Biological activityThe biological activity is determined by the inhibitory effect on the invasion of Mel In Tumor cells and found active in Mel In assay.
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Molecular Weight12.2 kDa
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Storage Temp-20°C
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ShippingBlue ice
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionSterile filtered and lyophilized with no additives
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Reconstitution InstructionsReconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers
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Background InformationMIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extraCellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis. Recombinant human MIA is a 12.2 kDa globular protein containing 108 amino acid residues including two intramolecular disulfide bonds.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMIA-RAB4B, MIA
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Short nameMIA, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameMIA, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameMIA-RAB4B readthrough (NMD candidate)
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Locus
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Discovery year2013-05-10
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMIA SH3 domain containing
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Synonyms gene name
- melanoma inhibitory activity
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
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Classification
- MIA family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data