HCC1937 Cell Line

• Catalog number
  EP-CL-0093
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  HCC1937
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  23 years adult
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The tumor was classified as TNM Stage ⅡB, grade 3BRCA1 analysis revealed that the cell line is homozygous for the BRCA1 5382C mutation, whereas the lymphoblastoid cell line derived from the same patient is heterozygous for the same mutationThis mutation was present in two other family members; an identical sister also developed breast cancerThe cell line has an acquired mutation of TP53 with wild-type allele loss; an acquired homozygous deletion of the PTEN gene, and loss of heterozygosity at multiple loci known to be involved in the pathogenesis of breast cancerThe cells are negative for expression of Her2-neu and for expression of p53. HCC1937 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2(EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor(ER) and progesterone receptor(PR).
• Biosafety
  1
• Medium
  RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  Estrogen receptor, negative; progesterone receptor, negative
• Antigen_expression
  NA
• Gene_expression
  Epithelial Glycoprotein 2(Egp2); Cytokeratin 19
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  HCC1937   Line  
• Short name
  HCC1937 Line
• Species
  Homo sapiens, Human
• Alternative name
  HCC1937 cellular Line
• Tissue
  Mammary gland, breast/duct

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