Rabbit anti Goat IgG (heavy and light chains), conjugated with FITC

• Catalog number
  MBS571147
• Price
  Please ask
• Size
  5x2 mL

• Products_type
  Secondary Antibody
• Products_short_name
  [Rabbit anti Goat IgG (heavy and light chains)]
• Clonality
  Polyclonal
• Reactivity
  Caprine
• Specificity
  Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Goat IgG, heavy and light chains.
• Storage_stability
  Store at 4 degree C.
• Tested_application
  ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus, Oryctolagus cuniculus
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

• Gene target
  heavy   light   chains   conjugated   with  
• Short name
  Rabbit anti Goat IgG (heavy light chains), conjugated with FITC
• Technique
  Rabbit, light, anti, FITC, Goat, IgG, antibody to, antibody Conjugates, Fluorescein, goats, IgGs
• Host
  Rabbit, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
• Isotype
  IgG, IgG
• Label
  FITC
• Species
  Goats
• Alternative name
  production species: rabbit antibody to caprine Immunoglobulin G (heavy and light chains), coupled including fluorecein
• Alternative technique
  rabbit-anti, antibodies, caprine, fluorescine, immunoglobulins

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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