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Gene info
Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.
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Gene names
CKb-6, MPIF-2, Scya24, C-C motif chemokine 24, Eosinophil chemotactic protein 2,
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Antibodie s label
unlabelled serum
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Purity
Anti-murine Eotaxin-2 specific antibody was purified by affinity chromatography employing immobilized murine Eotaxin-2 matrix.
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Ab raised in
Goat
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Type
Polyclonal
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Antigen
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant murine Eotaxin-2.
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Immuno application
ELISA, Western Blot, NELISAut
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Antibody advice
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mEotaxin-2 (100 ng/mL), a concentration of 7.0-8.0 ug/mL of this antibody is required. ELISA:To detect murine Eotaxin-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine Eotaxin-2.Sandwich: To detect mEotaxin-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Eotaxin-2 (XP-5125Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2. Western Blot:To detect murine Eotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine Eotaxin-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Keep at
Eotaxin-2 antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
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Works with
Mouse
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Omim nr
13431902
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Ncbi nr
Q9JKC0
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Vial with antibody
Lyophilized
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NCBI GENE nr
56221
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GENE symbol
Ccl24
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Antigene name
chemokine (C-C motif) ligand 24
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Source
Mus musculus
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Protein nr
Q9JKC0
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps