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Immunogen
Synthesized peptide derived from human GLUT1.
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Antibody s target
Human GLUT1
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Antibody s full description
Rabbit Anti-Human GLUT1 Polyclonal
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Primary research fields
Cancer, Cardiovascular System, Atherosclerosis
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) Antibody, CSE Antibody, DYT17 Antibody, DYT18 Antibody, DYT9 Antibody, EIG12 Antibody, erythrocyte/brain Antibody, Erythrocyte/hepatoma glucose transporter Antibody, facilitated glucose transporter member 1 Antibody, Glucose transporter 1 Antibody, Glucose transporter type 1 Antibody, Glucose transporter type 1, erythrocyte/brain Antibody, GLUT Antibody, GLUT-1 Antibody, GLUT1 Antibody, GLUT1DS Antibody, GLUTB Antibody, GT1 Antibody, GTG1 Antibody, Gtg3 Antibody, GTR1_HUMAN Antibody, HepG2 glucose transporter Antibody, HTLVR Antibody, Human T cell leukemia virus (I and II) receptor Antibody, MGC141895 Antibody, MGC141896 Antibody, PED Antibody, RATGTG1 Antibody, Receptor for HTLV 1 and HTLV 2 Antibody, SLC2A1 Antibody, Solute carrier family 2 (facilitated glucose transporter), member 1 Antibody, Solute carrier family 2 Antibody, Solute carrier family 2, facilitated glucose transporter member 1 Antibody
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Verified applications
WB, IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~54kDa, endogenous levels of total GLUT1 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1295 was sufficient for detection of GLUT1 in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane, Melanosome
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Tissue specificity
Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.
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Scientific context
Glucose transporter 1 is a uniporter protein that in humans is encoded by the SLC2A1 gene, also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1). GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier, whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.
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Bibliography
1. Mueckler M. et al. (1985) Science 229 (4717): 941–5. 2. Olson A.L. and Pessin J.E. (1996) Annual Review of Nutrition 16: 235–56.
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Released date
6/Sep/2016
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NCBI number
NP_006507.2
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Gene number
6513
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Protein number
P11166
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Tissue: Brain Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human Jurkat cell lysates showing detection of ~55kDa GLUT1 protein using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Lane 1: Human Jurkat cells. Lane 2: Human Jurkat cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:1000. Predicted/Observed Size: ~55kDa. Rabbit Anti-GLUT1 Antibody used in Immunohistochemistry (IHC) on Brain Tissue (SPC-1295) | Rabbit Anti-GLUT1 Antibody used in Western blot (WB) on Jurkat cell lysates (SPC-1295)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
