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Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr383 of human Chk2 (AA380-386)
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Antibody s target
Human Chk2 (pThr383)
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Antibody s full description
Rabbit Anti-Human Chk2 (pThr383) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Cds1 homolog Antibody, Checkpoint kinase 2 Antibody, CHEK 2 Antibody, CHK2 checkpoint homolog Antibody, CHK2_HUMAN Antibody, hCds1 Antibody, LFS 2 Antibody, PP1425 Antibody, RAD 53 Antibody, Serine/threonine protein kinase Chk2 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 60.915 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-950 was sufficient for detection of Chk2 (pThr383) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Chk2 is a protein kinase involved in cell cycle arrest in response to DNA damage. CHK2 is activated through phosphorylation by ATM and ATR at T68. Activated CHK2 phosphorylates and inhibits CDC25 a dual-specificity phosphatase required for progression through the cell cycle. CHK2 further causes cell cycle earrest through activation of a DNA repair protein BRCA1 and apoptosis factor PML. The role of CHK2 in the development of cancer is in a primarily tumour suppressor role through causing cell cycle arrest. It has been observed that inhibition of CHK2 through mutations in the regulatory FHA domain has been observed in low frequency in osteosarcomas and cancers of the ovary, lung and vulva. Another mutation, "1100delC" has been commonly found in breast cancers.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_009125
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Gene number
11200
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Protein number
O96017
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus