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Availability
Please contact us to check the availability
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Immunogen
Partially purified chicken vitamin D receptor
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Specificity
Vitamin D receptor, detects epitope aa 89-105 in human VDR
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Species reactivity
Mammals, birds, other vertebrates not tested
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Product presentation
Diluted ascites containing 0.01% sodium azide
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Product category
Primary Antibody
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Antibody s raised in
Rat
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Antibody s isotype
IgG2b
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Antibody s clone
1G2
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:500-1:1000 (IHC), 1:1000-1:2000 (WB)
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Protein concentration
N/A
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Positive control
Intestine, skin, kidney
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Pretreatment
Bouin's fixative recommended. Over-fixation will destroy the epitope. For antigen unmasking citrate buffer (ref. DE000) is suitable.
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Tested applications
IHC(P), IP, WB, functional inhibition (azide-free solution required!)
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Recommended secondary reagent
As a secondary reagent we recommend a biotinylated antibody against rat IgG (ref. BA-4000 or BA-4001 in combination with VECTASTAIN ABC Standard ref. PK-6100 and a suitable peroxidase substrate e.g. DAB (ref. DA002) or AEC (ref. AE002).
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Storage method
Store at -20°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Nemere I, Ray R, McManus W. (2000) Immunochemical studies on the putative plasmalemmal receptor for 1, 25(OH)(2)D(3). I. Chick intestine. Am J Physiol Endocrinol Metab. 2000 Jun;278(6):E1104-14 2. Bischoff HA, Borchers M, Gudat F, Duermueller U, Theiler R, Stahelin HB, Dick W. (2001) In situ detection of 1,25-dihydroxyvitamin D3 receptor in human skeletal muscle tissue. Histochem J. 2001 Jan;33(1):19-24 3. Balmain N, Hauchecorne M, Pike JW, Cuisinier-Gleizes P, Mathieu H. (1993) Distribution and subcellular immunolocalization of 1,25-dihydroxyvitamin D3 receptors in rat epiphyseal cartilage. Cell Mol Biol (Noisy-le-grand). 1993 May;39(3):339-50
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Tips
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
