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Category
Primary Antibodies
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Long description
The c-kit proto-oncogene encodes a transmembrane receptor with tyrosine kinase activity, c-kit (CD117), also known as stem cell factor receptor (SCFR), which is closely related to the platelet-derived growth factor receptor family. The c-kit oncoprotein plays a role during normal hematopoiesis, gametogenesis and melanogenesis. It is expressed in among others normal breast epithelium, melanocytes, mast cells and glial cells. Defects in c-kit are a cause of several diseases, such as gastrointestinal stromal tumor (GIST), and testicular germ cell tumor. Also acute myelogenous leukemia (AML), a malignant disease in which hematopoietic precursors are arrested in an early stage of development, is related to mutation in c-kit. As a result, the antibody to CD117 is suitable the characterization of certain leukemias.
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Antibody come from
T595 is a mouse monoclonal IgG1 antibody derived by fusion of p3-NS1-Ag4-1 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with prokaryotic recombinant protein corresponding to the three N-terminal C2-like extracellular domains of the human c-kit oncoprotein.
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Other description
Each vial contains 1 ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
T595
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Antigen antibody binding interaction
Mouse anti Human c-kit Oncoprotein (CD117) (T595) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
The antibody T595 recognises c-kit oncoprotein in human cells and tissues. The antibody reacts with the region corresponding to the three N-terminal C2-like extracellular domains of human c-kit.
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Research interest
Stem cell research, Cancer
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Application
Immunocytochemistry, Immunohistochemistry (frozen & paraffin)
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Antibody s suited for
The T595 antibody is suitable for the detection of the c-kit protein by immunocytochemistry on isolated cells, and immunohistochemistry on frozen and formalin fixed paraffin embedded tissues. For the latter application the high temperature antigen unmasking technique with 1 mM EDTA (pH 8.0) should be used (two cycles of 10 minutes in a microwave oven at 500 W ; see reference 5), and incubation with the primary antibody for 60 min. at 25 °C. Optimal antibody dilutions for the different applications should be determined by titration; recommended dilution 1:20 -1:40.
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Storage
Store undiluted antibody at 2-8ºC (until expiry date). For prolonged storage: store aliquots at -20ºC.
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Relevant references
1. Natali, P.G., Nicotra, M.R., Winkler, A.B., et al. (1992). Progression of human cutaneous melanoma is associated with loss of expression of c-kit proto-oncogene receptor. Int. J. Cancer 52, 197-201. 2. Matsuda, R., Takahashi, T., Nakamura, S., et al. (1993). Expression of the c-kit protein in human solid tumors and in corresponding fetal and adult normal tissues. Am. J. Pathol. 142, 339-46. 3. Izquierdo M.A., Van der Valk, P., Van Ark-Otte, J. et al. (1995). Differential expression of the c-kit proto-oncogene in germ cell tumors. J Pathol 177, 253-58. 4. Lasota, J., Miettinen, M. (2008). Clinical significance of oncogenic KIT and PDGFRA mutations in gastrointestinal stromal tumours. Histopathology 53, 245-66. 5. Miliaras, D., Karasavvidou, F., Papanikolaou, A., Sioutopoulou, D. (2004). KIT expression in fetal, normal adult, and neoplastic renal tissues. J Clin Pathol 57, 463-6
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Protein number
P10721
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
