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Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr172 of human CDK4 (AA169-175)
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Antibody s target
Human CDK4 (pThr172)
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Antibody s full description
Rabbit Anti-Human CDK4 (pThr172) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
CDK4_HUMAN Antibody, Cell division kinase 4 Antibody, CMM 3 Antibody, Crk3 Antibody, Cyclin dependent kinase 4 Antibody, Melanoma cutaneous malignant 3 Antibody, p34 cdk4 Antibody, PSK J3 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 33.73 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-943 was sufficient for detection of CDK4 (pThr172) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus, Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
CDK4 is a protein-serine/threonine kinase, and a part of the protein kinase complex important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. CDK4 is responsible for the phosphorylation of retinoblastoma gene product (Rb) and mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumourigenesis of a variety of cancers, such as cutaneous malignant melanoma type 3 (CMM3).
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_000066
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Gene number
1019
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Protein number
P11802
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
RPE, CDK4
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Short name
Rabbit Anti- CDK4 (pThr172) Polyclonal: RPE
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
RPE
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman cyclin-dependent kinase 4 (pThr172) polyclonal: RPE
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
cyclin-dependent kinase 4, CMM3 and PSK-J3, CDK4 and IDBG-43642 and ENSG00000135446 and 1019, transferase activity, nuclei, Cdk4 and IDBG-194056 and ENSMUSG00000006728 and 102641469,12567
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