OxiSelect™ Nε-(carboxymethyl) lysine (CML) Competitive ELISA test kit, Trial Size

  • Catalog number
    STA-816-T
  • Price
    Please ask
  • Size
    32 assays
  • We send this reagant on
    Blue Ice
  • The temperatureerature for storage is
    Upon receipt, aliquot and store the CML-BSA Standard, CML Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
  • SDS or safty data sheet
    more (STA-816-T) GHS.pdf
  • Type of reagent
    Oxidative Stress/Damage
  • Related techniques
    Oxidative Stress/ Damage, Advanced Glycation End Products, CML Assays (N-epsilon-(Carboxymethyl) Lysine), CML Competitive ELISA
  • Use of the reagent
    Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect™ N-epsilon-(Carboxymethyl) Lysine Competitive ELISA test kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA test kit in which the plate is coated with a CML conjugate.  Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal.
  • Cellular use
    Detect CML as low as 50 ng/mL from a variety of samples
  • Protocol information
    CML-modified BSA included as standard
  • Reagent properties
    Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples
  • Living modified organisme in this reagent or LMO
    Not applicable
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,91
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Storage
    Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
  • Group
    Enzyme-Linked Immunosorbent Assay kits
  • Gene target
  • Gene symbol
    BCR
  • Short name
    OxiSelect™ Nε-(carboxymethyl) lysine (CML) Competitive ELISA test kit, Trial Size
  • Technique
    ELISA test, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    OxiSelect™ Nε-(carboxymethyl) lysine (CML) Competitive Enzyme-linked immunosorbent assay test reagent, Trial Size
  • Alternative technique
    elisas, tests, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
  • Identity
  • Gene
    BCR
  • Long gene name
    BCR activator of RhoGEF and GTPase
  • Synonyms gene
  • Synonyms gene name
    • breakpoint cluster region
    • BCR, RhoGEF and GTPase activating protein
  • Synonyms
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
    613
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • C2 domain containing
    • My-T-BRC complex
    • Dbl family Rho GEFs
    • Pleckstrin homology domain containing
  • VEGA ID
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note: An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
  • Tree numbers
    • E01.370.225.812.735.830
    • E05.200.812.735.830
    • E05.478.566.380.810
    • E05.478.566.639.810
    • E05.478.594.760.830
    • E05.601.470.380.810
    • E05.601.470.639.810
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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