Aldolase Activity Colorimetric Assay Kit

• Catalog number
  K665-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Absorbance (OD 450 nm) • Applications- - Measurement of Aldolase activity in various tissues/cells - Analysis of glycolysis and gluconeogenesis pathways
• Detection Method
  Absorbance (OD 450 nm)
• Applications
  • Measurement of Aldolase activity in various tissues/cells • Analysis of glycolysis and gluconeogenesis pathways
• Sample Type
  • Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: adherent or suspension cells • Human serum and plasma
• Features Benefits
  • Rapid, convenient and high-throughput adaptable • Kit can detect aldolase activity less than 0.1 mU
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Aldolase (Fructose-Bisphosphate Aldolase: EC 4.1.2.13) is an important enzyme for both glycolysis and gluconeogenesis. It catalyzes the reversible reaction of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate & dihydroxyacetone. There are 2 classes of Aldolase - class l: found in animal and plant tissues and class II: found in prokaryotes and lower eukaryotes. Class I Aldolase has 3 isozymes- Type A: found in muscle and red blood cells, Type B: found in liver and kidney and Type C: found in brain. Aldolase A deficiency leads to myopathy & hemolytic anemia. Muscle disease and liver injury can also cause increased serum aldolase. Accurate detection of aldolase activity is valuable for diagnostic and mechanistic studies. In BioVision’s Aldolase Activity Assay, aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm. This assay kit is simple, sensitive, and high-throughput adaptable. Detection limit: less than 0.1 mU of aldolase activity in a variety of samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Aldolase   Activity   Kit  
• Short name
  Aldolase Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Aldolase Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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