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ELISA s application
For quantitative measurement of rat RANK in cell culture supernatant and serum.
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Gene tested
rat RANK
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ELISA standard curve
Concentration(pg/ml) + 0 + 62.5 + 125 + 250 + 500 + 1000 + 2000 + 4000 O.D. + 0.026 + 0.095 + 0.159 + 0.283 + 0.555 + 1.105 + 1.810 + 2.381
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ELISA kit detection limits
62.5pg/ml to 4000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural and recombinant rat RANK
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);880;1954;2755;950;2160;3052 ELISA standard deviations 36.96;107.5;159.8;51.3;138.2;228.9 CV(%);4.2;5.5;5.8;5.4;6.4;7.5
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ELISA kit description
GENTAUR's rat RANK ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for RANK was used as capure antibodies on the ELISA test plate. The internal standards (NSO, Q30-P213) and your samples are measured with a biotin conjugated polyclonal from goat specific for RANK will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat RANK in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- rat RANK antibodies;1 ST0000-10;Lyophilized recombinant rat RANK standard;10ng/tube×2 AR1107;Biotinylated anti- rat RANK antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
/
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml rat RANK standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant or serum to the ELISA plate. Keep in mind that each rat RANK standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
15-20
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More gene info
Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.
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Antigen
NSO, Q30-P213
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Tumor necrosis factor receptor superfamily member 11A
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Gene name
tumor necrosis factor receptor superfamily, member 11a, NFKB activator
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Alternative
CD265;FEO;LOH18CR1;Loss of heterozygosity 18 chromosomal region 1;ODFR;OFE;OPTB7;Osteoclast differentiation factor receptor;OSTS;PDB2;Receptor activator of NF-KB;Receptor activator of nuclear factor kappa B;TNFRSF11A;TNR11_HUMAN;TRANCER;Tumor necrosis factor receptor superfamily member 11A;Tumor necrosis factor receptor superfamily member 11a activator of NFKB;Tumor necrosis factor receptor superfamily member 11a NFKB activator
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Gen protein identity
F1M8Z6
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Entrez nr
498206
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
TNFRSF11A
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Short name
RANK PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
Rat, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Rat, Rats
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Alternative name
Rat RANK PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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