Human CellExp™ Osteoprotegerin / TNFRSF11B, human recombinant
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Catalog number7514-20
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PricePlease ask
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Size20 ug
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SynonymsTNFRSF11B, OCIF, OPG, Osteoprotegerin
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Alternative_namesTNFRSF11B, OCIF, OPG, Osteoprotegerin
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Description≥95% pure, active, human cell expressed, human recombinant Osteoprotegerin / TNFRSF11B - acts as decoy receptor for TNFSF11/RANKL
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RecombinantYes
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SourceHEK293 cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activityMeasured by its binding ability in a functional ELISA. Immobilized rh TNFRSF11B at 10 ug/ml (100 ul/well) can bind human TNFSF11 Fc Chimera with a linear ranger of 4-256 ng/ml.
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Molecular WeightThis protein is fused with 6×His tag at the C-terminus, has a calculated MW of 44.4 kDa. The predicted N-terminus is Glu 22. DTT-reduced Protein migrates as 55 kDa due to glycosylation.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in PBS, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
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Background InformationTumor necrosis factor receptor superfamily member 11B (TNFRSF11B) is also known as Osteoclastogenesis inhibitory factor (OCIF), Osteoprotegerin (OPG). TNFRSF11B is a secreted homodimer protein, which can interact with TNFSF10 and TNFSF11. TNFRSF11B acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolTNFRSF11B, BTF3P11
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Short nameCellExp™ Osteoprotegerin / TNFRSF11B, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ Osteoprotegerin / tumor necrosis factor receptor superfamily, member 11b, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targettumor necrosis factor receptor superfamily, member 11b, OCIF and OPG and TR1, TNFRSF11B and IDBG-33675 and ENSG00000164761 and 4982, protein binding, Extracellular, Tnfrsf11b and IDBG-140472 and ENSMUSG00000063727 and 18383, TNFRSF11B and IDBG-644876 and ENSBTAG00000007423 and 523822
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Gene info
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Identity
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Gene
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Long gene nameTNF receptor superfamily member 11b
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Synonyms gene
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Synonyms gene name
- osteoprotegerin
- tumor necrosis factor receptor superfamily, member 11b
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-09-05
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor receptor superfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebasic transcription factor 3 pseudogene 11
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Synonyms gene
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Synonyms gene name
- basic transcription factor 3, like 1
- basic transcription factor 3-like 1, pseudogene
- basic transcription factor 3, like 1 pseudogene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-12-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data