Anti-Rat CD28 Biotin (Clone JJ319) (mouse IgG1)

  • Catalog number
    GEN520090
  • Price
    Please ask
  • Size
    500ug
  • Also known as
    CD28
  • Other names
    T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; N/A; N/A
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    CD28
  • Gene name synonims
    N/A
  • Other gene names
    CD28; CD28; N/A; N/A
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    Mouse IgG1
  • Clone
    JJ319
  • Host organism
    Mouse (Mus musculus)
  • Species reactivity
    Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    CD28 (Tp44; receptor for B7 family) (Rat CD28); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from Bioreactor supernatant via Protein G Chromatography))
  • Concentration
    Antibody Concentration: 0.1 miligram per 1 mililiter
  • Storage and shipping
    Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
  • Tested applications
    Immunohistochemistry (IHC)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Gene target
    CD28   JJ319   IgG1  
  • Gene symbol
    CD28
  • Short name
    Anti- CD28 (Clone JJ319) (mouse IgG1)
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, mouses
  • Host
    Rat
  • Isotype
    IgG1
  • Label
    Biotin
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    Anti-Anti-Rat CD28 Biotin (Clone JJ319) (mouse IgG1)
  • Alternative technique
    murine, antibodies
  • Alternative to gene target
    CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
  • Clone name
    JJ319
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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