Goat anti Donkey IgG H + L HRP

  • Catalog number
    43R-1030
  • Price
    Please ask
  • Size
    1 mg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Purified Donkey IgG (H&L)
  • Raised in
    Goat
  • Specificity
    This antibody reacts with heavy chains on Donkey IgG and light chains on all Donkey immunoglobulins.
  • Cross Reactivity
    No reactivity is observed to non-immunoglobulin Donkey serum proteins.
  • Conjugate Tag
    HRP
  • Grade Purity
    Grater than 95% pure
  • Method of Purification
    Affinity purified using solid phase Donkey IgG (H&L)
  • Source
    Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
  • Concentration
    1.0 mg/ml (lot specific)
  • Form Buffer
    Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.1% (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 1.1 ml of deionized water. Prepare fresh working dilution daily.
  • Storage
    Store lyophilized material at 2-8 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    Please refer to the datasheet
  • Usage Recommendations
    1:200-1:5000 ELISA/WB, 1:500-1:5000 IHC
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Latin name
    Equus africanus asinus, Capra aegagrus hircus
  • Properties
    HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
  • Conjugation
    HRP Conjugated
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Gene target
    -
  • Gene symbol
    CALD1, FUT1, L3MBTL2
  • Short name
    Goat anti IgG H + L
  • Technique
    anti, Goat, IgG, antibody to, goats, IgGs
  • Host
    Goat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    IgG, IgG
  • Label
    HRP
  • Species
    Donkeys, Goats
  • Alternative name
    Goat anti Donkey IgG (H + L) secondary antibody (HRP)
  • Alternative technique
    antibodies, caprine, immunoglobulins
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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