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Category
Primary Antibodies
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Long description
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over Mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from Human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
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Antibody come from
RL-ph2 is a Mouse monoclonal IgG2a, κ antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with isolated M13 phage coat proteins.
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Other description
Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
RL-ph2
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Antigen antibody binding interaction
Mouse anti M13 phage coat protein g8p Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Not applicable
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.
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Research interest
Microbiology
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Application
Affinity chromatography,Flow Cytometry,Immunocytochemistry,Western blotting
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Antibody s suited for
RL-ph2 is particularly useful for immunoblotting of sepaRated phage proteins, and is also suitable for detection of M13 phages in multistep immunoblotting procedures, immunocytochemistry, flow cytometry, affinity chromatography and ELISA. Optimal antibody dilution should be determined by titration; recommended range is 1:2 – 1:25 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:100 for immunoblotting applications.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
Meulemans, E. V., Nieland, L. J., Debie, W. H., Ramaekers, F. C., and van Eys, G. J. (1995). Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody, Hum Antibodies Hybridomas 6, 113-8. Meulemans, E. V., Slobbe, R., Wasterval, P., Ramaekers, F. C., and van Eys, G. J. (1994). Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody, J Mol Biol 244, 353-60.
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Protein number
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Gene
M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
