Donkey anti-Rat IgG (H&L) - Affinity Pure, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep IgG

  • Catalog number
    MBS687681
  • Price
    Please ask
  • Size
    5x1 mg
  • Products_type
    Secondary Antibody
  • Products_short_name
    [IgG (H&L)]
  • Products_name_syn
    [Donkey anti-Rat IgG (H&L) - Affinity Pure]
  • Reactivity
    Rat. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin rat serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep
  • Specificity
    Based on IEP, this antibody reacts with: heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins
  • Purity
    Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Rat IgG.
  • Form
    Clear, colorless liquid
  • Concentration
    1.50 mg/ml (E 1% at 280 nm = 13.0)
  • Storage_stability
    Store undiluted liquid at 2-8 degree C. For storage at -20 degree C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
  • Tested_application
    Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
  • Test
    A high affinity purification column was use to purify anti- IgG (H L) - Pure, min x w/ , , goat, , , , , mouse, rabbit IgG by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular. The ultrapure anti- IgG (H L) - Pure, min x w/ , , goat, , , , , mouse, rabbit IgG supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
  • Properties
    Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Latin name
    Equus africanus asinus, Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Pigs and the smaller guinea pigs are frequent used as models for humans. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Gene target
    min  
  • Short name
    anti- IgG (H L) - Pure, min x w/ , , goat, , , , , mouse, rabbit IgG
  • Technique
    affinity, Rabbit, anti-, Mouse, anti, Goat, Pure, IgG, antibody to, antibodies, goats, IgGs, mouses, pure products
  • Host
    Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
  • Isotype
    IgG, Heavy & Light chains, IgG
  • Species
    Sheep, Bos Taurus genes are available form the Bovine Genome database. Chickens, Donkeys, Goats, Guinea Pigs, Hamsters, Horses, Humans, Mouses, Pigs and Guinea pigs, Rats, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
  • Alternative name
    Donkey Antibody toRat Immunoglobulin G (heavy + light chain) - protein Pure, min x w/bovine, chicken, caprine, guinea swine, hamster, horse, H. sapiens, mouse, production species: rabbit and ovine Immunoglobulin G
  • Alternative technique
    rabbit-anti, murine, antibodies, purified, caprine, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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