Follistatin, mouse recombinant

• Catalog number
  4718-20
• Price
  Please ask
• Size
  20 ug

• Synonyms
  FS, activin-binding protein, FSH-suppressing protein (FSP)
• Alternative_names
  FS, activin-binding protein, FSH-suppressing protein (FSP)
• Description
  An autocrine glycoprotein involved in binding and bioneutralization of members of the TGF-β superfamily
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Purity
  ≥90%
• Biological activity
  The activity is determined by the dose-dependent neutralization of 7.5 ng/mL human Activin A. Complete neutralization is typically reached at less than 0.3 ug/mL.
• Results
  0.3 ug/mL
• Molecular Weight
  31.6 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Recombinant mouse Follistatin is lyophilized from 10 mM Na2PO4 + 50 mM NaCl, pH 7.5.
• Reconstitution Instructions
  Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
• Background Information
  Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-beta family members (activin, BMPs, myostatin, GDF-11 and TGF-beta 1). Mechanistically, follistatin works as an antagonist by complexing with TGF-beta family members to prevent them from interacting with their signaling receptors. Recombinant mouse follistatin is a nonglycosylated protein, containing 289 amino acids, with a molecular weight of 31.6 kDa.
• Amino acid sequence
  MGNCWLRQAK NGRCQVLTKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DS
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Follistatin  
• Gene symbol
  FST, FSTL1, FSTL3
• Short name
  Follistatin, mouse recombinant
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  mouse
• Species
  Mouse, Mouses
• Alternative name
  Follistatin, mouse Rec.
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:3971
• Gene
  FST
• Long gene name
  follistatin
• Synonyms
  • FS
• GenBank acession
  • M19481
• Locus
  5q11.2
• Discovery year
  1999-09-07
• Entrez gene record
  10468
• Pubmed identfication
  • 10411917
  • 3380788
• RefSeq identity
  • NM_013409
• VEGA ID
  OTTHUMG00000131183

Gene info

• Identity
  HGNC:3972
• Gene
  FSTL1
• Long gene name
  follistatin like 1
• Synonyms gene name
  • follistatin-like 1
• Synonyms
  • FRP
  • FSL1
  • OCC1
  • OCC-1
  • tsc36
• GenBank acession
  • U06863
• Locus
  3q13.33
• Discovery year
  1999-09-07
• Entrez gene record
  11167
• Pubmed identfication
  • 7957230
  • 9786430
• RefSeq identity
  • NM_007085
• Classification
  • MicroRNA protein coding host genes
  • SPARC family
• VEGA ID
  OTTHUMG00000159440

Gene info

• Identity
  HGNC:3973
• Gene
  FSTL3
• Long gene name
  follistatin like 3
• Synonyms gene name
  • follistatin-like 3 (secreted glycoprotein)
• Synonyms
  • FLRG
  • FSRP
• Synonyms name
  • follistatin-related protein
• GenBank acession
  • U76702
• Locus
  19p13.3
• Discovery year
  1999-09-07
• Entrez gene record
  10272
• Pubmed identfication
  • 9671416
  • 15527507
• RefSeq identity
  • NM_005860
• VEGA ID
  OTTHUMG00000180593

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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