Biotin, Biotin carboxyl carrier subunit of chloroplast ACCase

• Catalog number
  AS15 2881
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Lyophilized
• Immunogen
  Purified native protein from Pisum sativum
• Raised in
  Guinea pig
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  AS15 2880 | anti-ACCase subunit beta | acetyl-coenzyme A, carboxylase (subunit beta) rabbit antibodySecondary antibodies
• Recommended dilutions for use
  1:2000 with ECL (WB)
• Molecular weight expected аpparent
  29.6 kDa
• Verified reactivity
  Arabidobsis thaliana, Hordeum vulgare, Pisum sativum, Solanum lycopersicum
• Possible reactivity
  Hordeum vulgare, Nicotiana tabacum
• No reactivity
  no confirmed exceptions from predicted reactivity are currently known
• Supplementary information
  Higher protein load of 30-50 µg/well is needed for other species than Arabidopsis thaliana.
• References
  to be added when available, antibody released in December 2015
• Scientific context
  Biotin carboxyl carrier subunit of chloroplast ACCase is a biotin carboxyl-carrier subunit of the multi-enzyme plastidial acetyl-coenzyme A carboxylase complex involved in fatty acid biosynthesis process.
• Notes
  None
• Protein number
  F4KE21
• TAIR number
  AT5G16390
• Hyperlink
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Description
  A carrier can hold protected the subject it is supporting for the period needed by its application as stated by agisera.

• Gene target
  carboxyl   subunit   chloroplast   ACCase  
• Short name
  , carboxyl subunit of chloroplast ACCase
• Label
  Biotin
• Alternative name
  biotinilated, biotinilated carboxyl carrier functionnal sequence on chloroplast ACCase

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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