Available ordering format Lyophilized
Recommended dilutions for use 1:2000 with ECL (WB)
Raised in Guinea pig
Storage condition For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Molecular weight (expected | аpparent) 29.6 kDa
Immunogen Purified native protein from Pisum sativum
Protein number F4KE21
Possible reactivity Hordeum vulgare, Nicotiana tabacum
Connected products AS15 2880 | anti-ACCase subunit beta | acetyl-coenzyme A, carboxylase (subunit beta) rabbit antibodySecondary antibodies
Scientific context Biotin carboxyl carrier subunit of chloroplast ACCase is a biotin carboxyl-carrier subunit of the multi-enzyme plastidial acetyl-coenzyme A carboxylase complex involved in fatty acid biosynthesis process.
TAIR number AT5G16390
No reactivity no confirmed exceptions from predicted reactivity are currently known
Verified reactivity Arabidobsis thaliana, Hordeum vulgare, Pisum sativum, Solanum lycopersicum
References to be added when available, antibody released in December 2015
How to reconstitute See included datasheet or contact our support service
Supplementary information Higher protein load of 30-50 µg/well is needed for other species than Arabidopsis thaliana.
Verified applications western blot (WB)
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Description A carrier can hold protected the subject it is supporting for the period needed by its application as stated by agisera.