This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Alternative names of antibody target:
BMK1 , ERK-5 , PRKM7 , MAPK7, Mitogen-activated protein kinase 7; MAP kinase 7; MAPK 7; Extracellular signal-regulated kinase 5; ERK-5; Big MAP kinase 1; BMK-1.
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~115 kDa Erk5 of human, mouse, and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Human, mouse, rat
Clonality of antibody:
Synthetic peptide from human Erk5
How to handle this antibody:
The antibody solution should be gently mixed before use.
Erk-5 shares high homology in the amino-terminal kinase domain with Erk1/2. However, the long carboxy-terminal kinase domain makes Erk5 a unique MAP kinase. Erk5 is activated by a variety of extracellular signals. The direct upstream kinase of Erk5 is defined as MEK5. Studies show that Erk5 pathway is involved in proliferation, differentiation, oncogenesis, and development.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.