FITC-Linked Polyclonal Antibody to Semaphorin 3A (SEMA3A)
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Catalog number
MBS2083379
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Price
Please ask
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Size
0,1 mg
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Other size
please contact us to order other different size
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
SEMA3A
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Short name
FITC-Linked Polyclonal Antibody Semaphorin 3A (SEMA3A)
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Technique
Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
FITC
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Alternative name
fluorecein-Linked polyclonal (antibody to-) to Semaphorin 3A (sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A)
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Alternative technique
polyclonals, antibodies, fluorescine
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Alternative to gene target
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, coll-1 and COLL1 and HH16 and Hsema-I and Hsema-III and SEMA1 and SEMAD and SEMAIII and SEMAL and SemD, SEMA3A and IDBG-24546 and ENSG00000075213 and 10371, chemorepellent activity, Extracellular, Sema3a and IDBG-135492 and ENSMUSG00000028883 and 20346, SEMA3A and IDBG-630984 and ENSBTAG00000018133 and 537777
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Gene info
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Identity
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Gene
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Long gene name
semaphorin 3A
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Synonyms gene
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Synonyms gene name
- sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1999-06-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- Semaphorins
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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