PIP, PIP1;5) aquaporins (40 µg, ALP-conjugated)
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Catalog numberAS09 487-ALP
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PricePlease ask
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Size40 µg
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Available ordering formatLiquid in PBS pH 7.4, conjugated to ALP
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Immunogensynthetic peptide conserved in Arabidopsis thaliana: PIP1;1 P61837,At3g61430 PIP1;2 Q06611,At2g45960 PIP1;3 Q08733,At1g01620 , PIP1;4 Q39196, At4g00430, PIP1;5 Q8LAA6 At4g23400
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified serum
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionStore at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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Verified applicationswestern blot (WB)
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Connected productscollection of antibodies to plasma membrane proteins AS09 488 | anti-PIP2;1 | aquaporin PIP2;1AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporinsAS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2bAS09 491 | anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3Plant protein extraction bufferSecondary antibodies
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Recommended dilutions for use1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent30.68 | 28 kDa
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Verified reactivityArabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. "Biji Jarak ", Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea
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Possible reactivitydicots including: Brassica sp., Vicia faba, monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum, trees: Juglans regia, Populus tremula,
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationProtein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
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ReferencesRefer to PubMed
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Scientific contextPIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,
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NotesAntibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for plasma membrane isolation can be found here.
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Protein numberQ06611 , Q08733 , Q39196
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TAIR numberAT3G61430
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Gene target
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Gene symbolPDLIM3, ASRGL1, ATRNL1, SLPI, CCL27, ACD, PIP, PAK1IP1, PSTPIP1
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Short namePIP, PIP1 5) aquaporins (40 µg, ALP-conjugated)
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Techniqueantibody Conjugates
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Alternative namePIP, PIP1;5) aquaporins (40 µg, ALP-coupled)
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Gene info
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Identity
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Gene
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Long gene namePDZ and LIM domain 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
- LIM domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameasparaginase and isoaspartyl peptidase 1
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Synonyms gene name
- asparaginase like 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-08-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameattractin like 1
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Synonyms gene name
- attractin-like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-03-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesecretory leukocyte peptidase inhibitor
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Synonyms gene name
- secretory leukocyte protease inhibitor (antileukoproteinase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-24
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Entrez gene record
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Pubmed identfication
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Classification
- WAP four-disulfide core domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 27
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 27
- chemokine (C-C motif) ligand 27
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-07-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameACD shelterin complex subunit and telomerase recruitment factor
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Synonyms gene name
- adrenocortical dysplasia homolog (mouse)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-01-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Shelterin complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameprolactin induced protein
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Synonyms gene name
- prolactin-induced protein
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Synonyms
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Synonyms name
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Locus
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Discovery year1988-08-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePAK1 interacting protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-06-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WD repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameproline-serine-threonine phosphatase interacting protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- F-BAR domain containing
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VEGA ID
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Locus Specific Databases