Modification No modification has been applied to this antibody
Clonality Polyclonal Antibody
Other name Anti-CAPON Polyclonal
Host organism Rabbit (Oryctolagus cuniculus)
Background information CAPON (carboxy-terminal PDZ ligand of nNOS) selectively binds within the 100 amino acid PDZ domain of the neuronal nitric oxide synthase (nNOS), but not to endothelial NOS or inducible NOS, and sequesters nNOS in the cytosol. Biosynthesis of the neurotransmitter nitric oxide (NO) requires the association of nNOS with various synaptic proteins, including syntrophin, postsynaptic density (PSD)95 and PSD93 through a scaffolding PDZ domain. These proteins facilitate the transport of nNOS to the plasma membrane, where it is catalytically activated by NMDA-receptor mediated calcium channels. The association of nNOS with PSD95 or PSD93 is regulated by CAPON. The carboxy terminus of CAPON binds to the PDZ domain, competes with PSD95 and PSD93 for binding to nNOS and in turn prevents the translocation and catalytic activation of nNOS.
Also known as CAPON Polyclonal Antibody
Swiss Prot N/A
Specificity This antibody reacts specifically with CAPON
Modification site None
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Conjugated with ALEXA FLUOR® 594
Gene ID N/A
Clone Polyclonal Antibodies
Cross Reactive Species details No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Synonyms NOS1AP; C terminal PDZ domain ligand of neuronal nitric oxide synthase CAPON; C terminal PDZ domain ligand of neuronal nitric oxide synthase; C terminal PDZ ligand of neuronal nitric oxide synthase protein; C-terminal PDZ ligand of neuronal nitric oxide synthase protein; CAPON; CAPON_HUMAN; Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein; Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein; Ligand of neuronal nitric oxide synthase with carboxyl terminal PDZ domain; MGC138500; Nitric oxide synthase 1 neuronal adaptor protein; Nitric oxide synthase 1 adaptor protein.
Subcellular locations N/A
Concentration 1ug per 1ul
Purification method Purified by Protein A.
Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Type Conjugated Primary Antibody
Cross reactive species Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Excitation emission 590nm/617nm
Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Target Protein/Peptide CAPON
Antigen Source KLH conjugated synthetic peptide derived from human CAPON
Properties For facs or microscopy Alexa 1 conjugate.
Conjugation Alexa Fluor,ALEXA FLUOR® 594
Conjugated Alexa conjugate 1
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.