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Immunogen
Synthesized peptide derived from C-terminal of human SLC6A8.
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Antibody s target
Human SLC6A8
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Antibody s full description
Rabbit Anti-Human SLC6A8 Polyclonal
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Primary research fields
Cell Signaling
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
SLC6A 8 Antibody, Creatine transporter 1 Antibody, CRTR Antibody, CT1 Antibody, Sodium and chloride-dependent creatine transporter 1 Antibody, Solute carrier family 6 member 8 Antibody
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Verified applications
WB, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~70kDa, endogenous levels of total SLC6A8 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1333 was sufficient for detection of SLC6A8 in 10 µg of HuvEc cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
Predominantly expressed in skeletal muscle and kidney. Also found in brain, heart, colon, testis and prostate.
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Scientific context
SLC6A8 is required for the uptake of creatine in muscles and brain. Deficiency of this gene is an X-linked form of intellectual diavility and is the most common casue of cerebral creatine syndromes.
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Bibliography
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Released date
6/Sep/2016
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NCBI number
NP_005620.1
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Gene number
6535
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Protein number
P48029
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human HuvEc cell lysates showing detection of ~70kDa SLC6A8 protein using Rabbit Anti-SLC6A8 Polyclonal Antibody (SPC-1333). Lane 1: Human HUVEC cells. Lane 2: Human HUVEC cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-SLC6A8 Polyclonal Antibody (SPC-1333) at 1:1000. Predicted/Observed Size: ~70kDa. Rabbit Anti-SLC6A8 Antibody used in Western blot (WB) on HuvEc cell lysates (SPC-1333)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus