ATF2 Antibody (pThr73): FITC

• Catalog number
  SPC-1347D-FITC
• Price
  Please ask
• Size
  100 µl

• Target
  ATF2 (pThr73)
• Conjugate
  FITC
• Research Area s
  Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Domain Families, Hlh, Immunology, Innate Immunity, TLR Signaling
• Alternative Name s
  ATF2 Antibody, Histone acetyltransferase ATF2 Antibody, DKFZP727M111 Antibody, Cyclic AMP-responsive element-binding protein 2 Antibody, cAMP response element-binding protein CRE-BP1 Antibody, EC:2.3.1.48 Antibody, cAMP-responsive element-binding protein 2 Antibody, HB16 Antibody, FLJ12732 Antibody, CREB-2 Antibody, cAMP-dependent transcription factor ATF2 Antibody, KIAA1403 Antibody, Rpap1 Antibody, Cyclic AMP-dependent transcription factor ATF2 Antibody, RNA polymerase II-associated protein 1 Antibody, MGC858 Antibody, CREB2 Antibody, RNA polymerase II associated protein 1 Antibody, Activating transcription factor 2 Antibody, RPAP1_HUMAN Antibody, CREBP1 Antibody
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic phospho-peptide from human ATF2 around the phosphorylation site of threonine 73 (pThr73).
• Immunogen Species
  Human
• Accession Number
  NP_001243019.1
• Gene ID
  1386
• Swiss Prot
  P15336
• Applications
  WB, IHC, IP, ELISA
• Host Species
  Rabbit
• Species Reactivity
  Human, Mouse, Rat
• Antibody Dilution
  WB (1:250-1:1000); IHC (1:50-1:150); IP (1-2.5 ug per mg lysate); ELISA (1:10000); optimal dilutions for assays should be determined by the user.
• Purification
  Peptide Affinity Purified
• Storage Buffer
  PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
• Specificity
  Detects endogenous levels of ATF2 protein only when phosphorylated at threonine 73 (pThr73). Detects a band at ~52 kDa.
• Storage Temperature
  -20ºC
• Shipping Temperature
  Blue Ice or 4ºC
• Cellular Localization
  Mitochondrion Outer Membrane, Nucleus, Cytoplasm, Mitochondrion, Nucleoplasm, Site Of Double-Strand Break
• Field of Use
  For in vitro research use only.
• Technical data
• SDS
• Gross Weight kg
  1.4
• Net Weight g
  0.1
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  ATF2   pThr73  
• Gene symbol
  GDNF, ATF2
• Short name
  ATF2 Antibody (pThr73): FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  ATF2 (antibody to-) (pThr73): fluorecein
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:4232
• Gene
  GDNF
• Long gene name
  glial cell derived neurotrophic factor
• Synonyms
  • ATF1
  • ATF2
  • HFB1-GDNF
• Synonyms name
  • astrocyte-derived trophic factor
  • glial cell line derived neurotrophic factor
  • glial derived neurotrophic factor
• Locus
  5p13.2
• Discovery year
  1995-05-04
• Entrez gene record
  2668
• Pubmed identfication
  • 8493557
• RefSeq identity
  • NM_000514
• Classification
  • GDNF family ligands
• VEGA ID
  OTTHUMG00000090809

Gene info

• Identity
  HGNC:784
• Gene
  ATF2
• Long gene name
  activating transcription factor 2
• Synonyms gene
  • CREB2
• Synonyms gene name
  • cAMP responsive element binding protein 2
• Synonyms
  • TREB7
  • CRE-BP1
  • HB16
• GenBank acession
  • X15875
• Locus
  2q31.1
• Discovery year
  1991-08-01
• Entrez gene record
  1386
• Pubmed identfication
  • 1833307
  • 1838349
• RefSeq identity
  • NM_001880
• Classification
  • Basic leucine zipper proteins
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000132424

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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