Luciferase Reporter Assay Kit

• Catalog number
  K801-200
• Price
  Please ask
• Size
  200 assays

• Description
  Highly Sensitive Assay
• Summary
  • Detection method- Luminescence • Species reactivity- Mammalian • Application- This Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells.
• Sample Type
  Cell and tissue culture homogenates (eukaryotic cells)
• Features Benefits
  • Simple procedure; takes ~ 20-30 minutes • Fast and convenient • The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer. BioVision’s Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, mg²ᶧ and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash Kinetics). The luminescent reaction is then triggered by an injection of luciferin, and the emitted light is recorded by a luminometer. By providing CoA, along with ATP, mg²ᶧ in an optimized buffer solution, BioVision’s Luciferase Reporter Assay Kit ensure maximal sensitivity, consistent light output, as well as convenience and consistency when working with multiple samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Luciferase   Reporter   Kit  
• Short name
  Luciferase Reporter Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Label
  luciferase
• Alternative name
  Luciferase detector test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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