NOX5 Antibody

• Catalog number
  R31049
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  NOX5
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the NOX5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This NOX5 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q96PH1
• Purity
  Antigen affinity
• Description
  NADPH Oxidase 5, also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. It is a NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al.(2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al.(2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells(HC), but not normal B cells.
• Immunogen
  An amino acid sequence from the C-terminus of human NADPH Oxidase 5 (KVLKGHCEKFGFRFFQENF) was used as the immunogen for this NOX5 antibody.
• Storage
  After reconstitution, the NOX5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  NOX5  
• Gene symbol
  NOX5
• Short name
  Anti-NOX5
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to NOX5
• Alternative technique
  antibodies
• Alternative to gene target
  NADPH oxidase, EF-hand calcium binding domain 5, NOX5 and IDBG-547502 and ENSG00000255346 and 79400, NADP binding, Plasma membranes, NOX5 and IDBG-645522 and ENSBTAG00000009030 and 531303

Gene info

• Identity
  HGNC:14874
• Gene
  NOX5
• Long gene name
  NADPH oxidase 5
• Synonyms gene name
  • NADPH oxidase, EF-hand calcium binding domain 5
• Synonyms
  • NOX5A
  • NOX5B
• GenBank acession
  • AF317889
• Locus
  15q23
• Discovery year
  2001-03-05
• Entrez gene record
  79400
• Pubmed identfication
  • 11483596
• RefSeq identity
  • NM_024505
• Classification
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000133320

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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