Rabbit Anti-Human Smad4 Polyclonal: Unconjugated

  • Catalog number
    SPC-1286D
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    Synthesized peptide derived from internal of human Smad4.
  • Antibody s target
    Human Smad4
  • Antibody s full description
    Rabbit Anti-Human Smad4 Polyclonal
  • Primary research fields
    Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic, Cardiovascular System, Heart, Cardiogenesis, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    (Small) Mothers Against Decapentaplegic Antibody, Deleted in Pancreatic Carcinoma 4 Antibody, DPC 4 Antibody, hSMAD4 Antibody, JIP Antibody, MADH 4 Antibody, Mothers against decapentaplegic homolog 4 Antibody, SMAD 4 Antibody, SMAD mothers against DPP homolog 4 Antibody
  • Verified applications
    WB, ELISA
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects ~60kDa, endogenous levels of total Smad4 protein.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-1286 was sufficient for detection of Smad4 in 10 µg of HeLa, Cos7, HepG2, HUVEC cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Nucleus
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Smad4 mediates all activities through interaction with various other Smad family members.
  • Bibliography
    1. Heldin C.H. et al. (1997) Nature. 390: 465-71. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-94. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-91. 4. Whitman M. (1998) Genes Dev. 12: 2445-62. 5. Wu G. et al. (2000) Science. 287: 92-7.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_005350.1
  • Gene number
    4089
  • Protein number
    Q13485
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure legend
    Western blot analysis of Human, Monkey HeLa, Cos7, HepG2, HUVEC cell lysates showing detection of ~60kDa Smad4 protein using Rabbit Anti-Smad4 Polyclonal Antibody (SPC-1286). Lane 1: Human HeLa. Lane 2: Monkey Cos7. Lane 3: Human HepG2. Lane 4: Human HUVEC. Lane 5: Treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Smad4 Polyclonal Antibody (SPC-1286) at 1:1000. Predicted/Observed Size: ~60kDa. Rabbit Anti-Smad4 Antibody used in Western blot (WB) on HeLa, Cos7, HepG2, HUVEC cell lysates (SPC-1286)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Conjugation
    not conjugated 
  • Gene target
  • Short name
    Rabbit Anti- Smad4 Polyclonal: Unconjugated
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG
  • Label
    Unconjugated
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman SMAD family member 4 polyclonal: nonconjugated
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
  • Alternative to gene target
    SMAD family member 4, DPC4 and JIP and MADH4 and MYHRS, SMAD4 and IDBG-3485 and ENSG00000141646 and 4089, transforming growth factor beta receptor, nuclei, Smad4 and IDBG-157503 and ENSMUSG00000024515 and 17128, SMAD4 and IDBG-644730 and ENSBTAG00000006919 and 540248
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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