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Alternative_name
Biotin-VAD-FMK , VAD-FMK, Biotin-
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Description
A synthetic peptide that irreversibly inhibits activity of caspase family proteases and blocks apoptosis. The inhibitor is designed as a methyl ester to enhance cell permeability. The conjugation with biotin allows detection of activated caspases in conjunction with avidin-tags. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
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Structure Available
No
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Peptide sequence
Biotin-Val-Ala-Asp(OMe)-FMK
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Salt Form
No
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Molecular Formula
C₃₀H₄₉FN₆O₈S
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Molecular Weight
672.81
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Cell Permeable
Yes
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Purity
>95% by HPLC
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Handling
Protect from light and moisture
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Tag Line
A pan-caspase inhibitor
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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PubChem CID
44135213
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SMILES
CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)CCCCCNC(=O)CCCCC1C2C(CS1)NC(=O)N2
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InChi
InChI=1S/C30H49FN6O8S/c1-17(2)26(29(43)33-18(3)28(42)34-19(21(38)15-31)14-25(41)45-4)36-24(40)12-6-5-9-13-32-23(39)11-8-7-10-22-27-20(16-46-22)35-30(44)37-27/h17-20,22,26-27H,5-16H2,1-4H3,(H,32,39)(H,33,43)(H,34, 42)(H,36,40)(H2,35,37,44)
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InChi Key
MOQLQMKJGYKHCT-UHFFFAOYSA-N
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MSDS Available
Yes
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
