Recombinant Human PRMT5 / SKB1 Protein (His & FLAG tag)

• Catalog number
  PKSH031276-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  HRMT1L5;IBP72;JBP1;SKB1;SKB1Hs
• Activity
  NA
• Sequence
  Ala 2-Leu 637
• Fusion tag
  C-His & N-FLAG
• Accession
  NP_006128.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞85 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  75 kDa
• AP Mol Mass
  65 kDa
• Formulation
  Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM EDTA, 0.5PMSF, 0.5mM, TCEP, 25% glycerol
• Background
  Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation, signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family, and exists as at least homodimers and homotetramers, or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development, as well as histone H3 'Arg-8', which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally, it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.
• Technical data
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Flag, histidine
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PRMT5   SKB1   Protein   His   tag  
• Gene symbol
  PRMT5, PRMT5-AS1, PRMT5-DT
• Short name
  Recombinant PRMT5 / SKB1 Protein (His & tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens PRMT5 / SKB1 Protein (histidine & FLAG detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:10894
• Gene
  PRMT5
• Long gene name
  protein arginine methyltransferase 5
• Synonyms gene
  • HRMT1L5
  • SKB1
• Synonyms gene name
  • skb1 (S. pombe) homolog
  • SKB1 homolog (S. pombe)
• Synonyms
  • SKB1Hs
• GenBank acession
  • AF015913
• Locus
  14q11.2
• Discovery year
  1999-06-18
• Entrez gene record
  10419
• Pubmed identfication
  • 9843966
• Classification
  • 7BS protein arginine methyltranferases
• VEGA ID
  OTTHUMG00000028709

Gene info

• Identity
  HGNC:40533
• Gene
  PRMT5-AS1
• Long gene name
  PRMT5 antisense RNA 1
• Synonyms gene name
  • PRMT5 antisense RNA 1 (non-protein coding)
• GenBank acession
  • AK093277
• Locus
  14q11.2
• Discovery year
  2011-11-18
• Entrez gene record
  100505758
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000149898

Gene info

• Identity
  HGNC:55482
• Gene
  PRMT5-DT
• Long gene name
  PRMT5 divergent transcript
• Locus
  14q11.2
• Discovery year
  2021-03-11
• Entrez gene record
  101926933
• Classification
  • Divergent transcripts

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products