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Synonyms
triosephosphate isomerase, TPI
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Alternative_names
TPI1 human Recombinant , Recombinant human TPI1, TPI1, TPI1 Recombinant, Human TPI1, Human Recombinant TPI1, TPI1, TPI1 Recombinant, Human TPI1, Human TPI1, human TPI1, TPI1 Recombinant protein, TPI1 human protein, Human TPI1 protein, Human TPI1 Recombinant protein, TPI1 protein, TPI1 protein, TPI1 Recombinant protein, triosephosphate isomerase, TPI
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Description
Involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis
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Recombinant
Yes
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Source
E. Coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Activity Specifications test method
Measured by the ability to convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.
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Biological activity
Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.
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Results
>3000 units/mg
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Unit Definition
One unit is the amount of enzyme which will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.
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Molecular Weight
28.8 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Concentration
0.5 mg/ml
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Appearance
Liquid
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Physical form description
In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT
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Background Information
TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Recombinant human TPI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
