HSP70 (C-terminus) monoclonal antibody

Alternative Name

Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2; HSPA1A; HSPA1; HSX70; HSPA1B; HSP-70; HSP 70
Immunogen

Synthetic peptide, corresponding to the C-terminus of Human HSP70.
Swiss Prot

P08107
Reactivity

Human
Applications

WB IP
Application_all

WB: 1:1000 IP: 1:50~1:200
Product

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity

This antibody detects endogenous levels of HSPA1A and does not cross-react with related proteins
Usage

Western blot detection of HSPA1A in Hela&HsHela cell lysates using HSP70 antibody (1:1000 diluted) .
BiowMW

Predicted band size:70KDa Observed band size:70KDa
Note

For research use only, not for use in diagnostic procedure.
Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
French translation

anticorps

Gene target

HSP70 monoclonal
Gene symbol

HSPA1B, HSPA14, HSPA1A, HSPA1L
Short name

HSP70 ( ) monoclonal antibody
Technique

Antibody, antibodies against human proteins, antibodies for, antibodies directed against c-terminus peptides can be suited for IHC because in the quaternary structure the c-terminus of this protein is often at the outside of the cell membrane. Monoclonals or monoclonal antibodies
Host

Mouse
Alternative name

HSP70 (C-terminus) monoclonal (antibody to-)
Alternative technique

antibodies

Identity

HGNC:5234
Gene

HSPA1L
Long gene name

heat shock protein family A (Hsp70) member 1 like
Synonyms gene name
- heat shock 70kD protein-like 1
- heat shock 70kDa protein 1-like
Synonyms
- HSP70-HOM
- hum70t
GenBank acession
- D85730
Locus

6p21.33
Discovery year

1989-05-12
Entrez gene record

3305
Pubmed identfication
- 9685725
- 9349405
Classification
- TIM23 complex
- Heat shock 70kDa proteins
VEGA ID

OTTHUMG00000031208

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data