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Category
Primary Antibodies
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Long description
NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be geneRated by alternative splicing and by posttranslational modifiCations, such as sialylation. During sialylation, polysialic acid (PSA) carbohydRates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.
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Antibody come from
NKI-NBl-1 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with neuroblastoma cell line CHP-212.
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Other description
Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
NKI-nbl-1
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Antigen antibody binding interaction
Mouse anti NCAM (CD56) Antibody
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Antibody is raised in
Mouse (Balb/c)
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
Different antibodies recognize a heterodimeric glycoprotein of 145, 185 kD which has been identified as NCAM (Neural Cell Adhesion Module). Two major epitopes have been defined, NKI-nbl-1 (art. nr. MUB1321S and MUB1321P) reacts with epitope 1 and 123C3 (art. nr. MUB1301S and MUB1301P) reacts with epitope 2.
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Research interest
Cancer,Neurobiology,Stem cell research, CD Markers
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Application
Immunofluorescence,Immunohistochemistry (frozen),Immunoprecipitation
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Antibody s suited for
NKI-nbl-1 is useful for immunofluorescence, immunoprecipitation and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
K. Moolenaar et al.Cancer Research 50, 1102-1106, Feb15, 1990.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
