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Stock availabilitIn Stock
In Stock
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CAS number
No Available. See included datasheet
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Description
FRET ratiometric fluorescence-based redox sensor
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Molecular weight
639 g/mol
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Primary research fields
Cancer, Oxidative Stress
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Other name
7-(Diethylamino)-N-((1r,4r)-4-(2-(10-ethyl-2,4-dioxo-4,10-dihydrobenzo[g]pteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide (FCR1)
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Product category
Small Molecules
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Product type
Redox Probe
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Chemical formula
C34H37N7O6
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Origin
Synthetic
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Purity pourcentage
Contact our support service
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Soluble in
Soluble in DMSO
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Physical appearance
Orange Solid
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Safety information
Classification: Caution: Substance not yet fully tested. Safety Phrases: S22 - Do not breathe dust S24/25 - Avoid contact with skin and eyes S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection
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PubChem number
Refer to PubChem
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Scientific context
FCR1 or flavin coumarin redox sensor 1, is a novel ratiometric fluorescent redox sensor utilized in fluorescence lifetime imaging microscopy and flow cytometry. In the oxidized form, excitation of FCR1 at 405nm results in a green fluorescence with max emissions at 525nm. Treatment with a mild reducing agent (including sodium cyanoborohydride, DTT, and glutathione) reduces the flavin thereby decreasing the green fluorescence intensity, and increasing the blue. Re-oxidation can be achieved using mild oxidizing agents. In general, the FCR1 probe can be used to observe changes in oxidative capacity without interference from background effects.
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Bibliography
1. Kaur A., Haghighatbin M.A., Hogan C.F., and New E.J. (2015) Chem. Commun. Epub.
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Release date
15-Jun-2015
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Representative figure link
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Representative figure legend
Chemical structure and design of FCR1 (SIH-180), showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3. | Fluorescence behavior of FCR1 (SIH-180) in the oxidized (green) and reduced (blue) forms, using 10 µM. Excitation: 405 nm. Reduced Emission: 475 nm. Oxidized Emission: 520 nm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3. | Two photon - confocal microscopy imaging of HeLa cells treated with FCR1 (SIH-180, 10 µM, 15 min, λex = 820 nm) and (a) N-acetyl cysteine (50 µM, 30 min), (b) vehicle control and (c) H2O2 (50 µM, 30 min) in blue and green channels. The pseudo colour ratio images indicate the ratio of emission intensity in the green channel to blue channel. Scale bar represents 20 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3. | Fluorescence lifetimes of the donor (420 – 470 nm) in HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 820 nm) and N-acetyl cysteine, vehicle control and H2O2. Pseudo-colour images represent mean lifetime. Scale bar represents 50 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3. | Flow cytometric studies of HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 405 nm) showing the fluorescence ratio (560 / 450 nm) when treated with N-acetyl cysteine (blue), vehicle control (green) and H2O2 (red). Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3. Chemical structure and design of FCR1, a FRET ratiometric fluorescence-based redox sensor (SIH-180). | Fluorescence behavior of FCR1 (SIH-180), a FRET ratiometric fluorescence-based redox sensor, in the oxidized and reduced forms. | Two photon - confocal microscopy imaging of HeLa cells treated with FCR1 (SIH-180), a FRET ratiometric fluorescence-based redox sensor. | Fluorescence lifetimes of the donor in HeLa cells treated with FCR1 (SIH-180), a FRET ratiometric fluorescence-based redox sensor. | Flow cytometric study of HeLa cells treated with FCR1 (SIH-180), a FRET ratiometric fluorescence-based redox sensor.
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
0.25
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Net weight g
0.001
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Test
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
