KMT1E/SETDB1 Antibody, ALEXA FLUOR 555

• Catalog number
  bs-11670R-A555
• Price
  Please ask
• Size
  100ul

• Long name
  KMT1E/SETDB1 Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
• Also known as
  Anti-KMT1E/SETDB1 PAb ALEXA FLUOR 555
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 555
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  KMT1E/SETDB1
• Specificity
  This is a highly specific antibody against KMT1E/SETDB1.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  210-260/1291
• Subcellular location
  Nucleus
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SETDB1
• Gene ID Number
  9869
• Swiss Prot
  Q15047
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  553nm/568nm
• Synonyms
  ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4; Histone-lysine N-methyltransferase SETDB1; ERG-associated protein with SET domain; Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SETDB1; KIAA0067
• Properties
  For facs or microscopy Alexa 1 conjugate. Very high photo stable ALEXA conjugate. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor, ALEXA FLUOR 555
• French translation
  anticorps

• Gene target
  KMT1E/SETDB1  
• Gene symbol
  SETDB1
• Short name
  Anti-KMT1E/SETDB1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 555
• Alternative name
  KMT1E/SETDB1 Polyclonal Antibody, ALEXA FLUOR 555
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10761
• Gene
  SETDB1
• Long gene name
  SET domain bifurcated histone lysine methyltransferase 1
• Synonyms gene name
  • SET domain, bifurcated 1
  • SET domain bifurcated 1
• Synonyms
  • KG1T
  • KIAA0067
  • ESET
  • KMT1E
  • TDRD21
• Synonyms name
  • tudor domain containing 21
• GenBank acession
  • D31891
• Locus
  1q21.3
• Discovery year
  1999-07-19
• Entrez gene record
  9869
• Pubmed identfication
  • 10343109
• RefSeq identity
  • NM_001145415
• Classification
  • Tudor domain containing
  • Methyl-CpG binding domain containing
  • SET domain containing
  • Lysine methyltransferases
• VEGA ID
  OTTHUMG00000035003

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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