Filters
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing

Mouse Anti Rat CaMKII Monoclonal IgG1 Clone 22B1

Mouse Anti Rat CaMKII Monoclonal IgG1 Clone 22B1 is available 20 times from Stressmark antibodies labs

SMC-125D-A700 | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 416.10 USD

Loading

SMC-125D-RPE | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 412.77 USD

Loading

SMC-125D-A390 | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 417.21 USD

Loading

SMC-125D-STR | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 413.88 USD

Loading

SMC-125D-A488 | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 416.10 USD

Loading

SMC-125D-A680 | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 416.10 USD

Catalog number SMC-125D-A680
Price 416.10 USD
Size 100 µg
Antibody's full description Mouse Anti-Rat CaMKII Monoclonal IgG1 Antibody, Clone: 22B1: ATTO 680
Net weight (g) 0.1
MeSH Data
Name Blotting, Western
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Product images
    #Mouse#.jpg#Rat#.png

SMC-125D | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 362.84 USD

Loading

SMC-125S | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 12 µg | 55.48 USD

Loading

SMC-125D-A565 | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 416.10 USD

Loading

SMC-125D-ALP | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 409.44 USD

Loading

SMC-125D-FITC | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 407.22 USD

Loading

SMC-125D-A633 | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 416.10 USD

Loading

SMC-125D-A594 | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 416.10 USD

Loading

SMC-125D-PCP | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 414.99 USD

Loading

SMC-125D-APC | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 414.99 USD

Loading

SMC-125D-A655 | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 416.10 USD

Loading

SMC-125D-P594 | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 423.87 USD

Loading

SMC-125D-BI | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 411.66 USD

Loading

SMC-125C | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 25 µg | 205.28 USD

Loading

SMC-125D-HRP | Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 size: 100 µg | 402.78 USD

Loading
Clone 22B1
Immunogen Synthetic peptide
Antibody's category Monoclonal Antibodies
Antibody's other name CSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody
Raised in Mouse
Antibody's target CaMKII
Primary research fields Cell Signaling, Post-translational Modifications, Phosphorylation
Brandname None
Antibodies' applications WB, IHC, ICC/IF, IP, ELISA
Antibody's reactivity Human, Mouse, Rat
Antibody's dilutions WB (1:1000), IHC (1:100), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Purity Protein G Purified
Antibody buffer for storage PBS pH7.4, 50% glycerol, 0.09% sodium azide
Antibody's concentration 1 mg/ml
Antibody's specificity Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
Storage recommendations -20ºC
Shipping recommendations Blue Ice or 4ºC
Antibody certificate of analysis 1 µg/ml was sufficient for detection of 0.2 µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Antibody in cell Cytoplasm , Mitochondrion , Nucleus , Cell Junction , Synapse , Presynaptic Cell Membrane
Tissue specificity See included datasheet or contact our support service
Scientific context CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Bibliography 1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167.
Released date 1-Nov-2007
PubMed number 20148318
Tested applications Western Blot
Tested reactivity Mouse
NCBI number NP_037052.1
Gene number 25400
Protein number P11275
Antibody's datasheet Contact our support service
Representative figure linkmore | details | see here | read more
Representative figure legend Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (SMC-125). Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (SMC-125) at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech. Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (SMC-125). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (SMC-125) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (SMC-125). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (SMC-125) at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (SMC-125). Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (SMC-125) at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK. Mouse Anti-CaMKII Antibody [22B1] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Rat dissociated hippocampal neurons (SMC-125) Mouse Anti-CaMKII Antibody [22B1] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-125) Mouse Anti-CaMKII Antibody [22B1] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-125) Mouse Anti-CaMKII Antibody [22B1] used in Western Blot (WB) on Mouse Ventricle lysates (SMC-125)
Warning information Non-hazardous
Country of production Canada
Total weight (kg) 1.4
Stock availability In Stock
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name Mus musculus,Rattus norvegicus
Gene targetCaMKII Monoclonal IgG1 22B1
Short name Mouse Anti- CaMKII Monoclonal IgG1, Clone 22B1
Technique Mouse, anti
Host mouse
Isotype IgG1
Species Rat
Alternative name Mouse antibody to-Rat CaMKII monoclonal IgG1, clonality 22B1
Alternative technique antibodies
Clone name 22B1
Similar products
No similar products groups has been found
Ask
Ajax processing
Close
Mouse Anti-Rat CaMKII Monoclonal IgG1, Clone 22B1 cat.: SMC-
Filters
+32-(0)1-658-90-45 [email protected]
  • CaMKII
  • Monoclonal
  • IgG1
  • 22B1
Back
Contact us
Ajax processing
Close
Chat with gentaur.com employee