Category Primary Antibodies
Species Reactivity Rat
Detection Range 1.56-100 ng/mL
Stock availability Shipped within 5-10 working days.
Shelf life Use Rat ELA4 (Elastase 4) ELISA Kit before 6 months
Raised in Mouse
Shipping Conditions Ice packs
Detection Method Colorimetric
Estimated Turnaround Time 6-11 business days
Immunogen see included datasheet or contact us
Species reactivity Human, Mouse, Rat (for other species please inquire)
Target Name NA
Quality Systems The kit is manufactured at ISO 9001 certified facilities.
Storage Temperature Short term: 4°C; Long term: see manual.
Purification Affinity purified
Applications WB (for other applications please contact us)
Footnote This product is for research use only.
Research Area Cancer, Cell Biology
Test Principle This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ELA4 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ELA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ELA4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ELA4. The concentration of Rat ELA4 in samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Type Sandwich
Sensitivity 0.94 ng/mL
Precaution of Use The Stop Solution is acidic. Do not allow to contact skin or eyes.
Sample Type Serum, Plasma, Biological Fluids
Cross Activity No significant cross-reactivity or interference was observed.
Storage conditions Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Target's alterntive name ELA4
Assay Time 4.5 hours
Application For research use only. Not for diagnostic procedures.