mPGES, PGES-1 glutathione dependent prostaglandin E synthase
-
Catalog numberAS03 031
-
PricePlease ask
-
Size100 µl
-
-
Available ordering formatLyophilized
-
ImmunogenRecombinant human purified 6-His mPGES-1 O14684
-
Raised inRabbit
-
ClonalityPolyclonal
-
ClonePolyclonal
-
PurificationSerum
-
How to reconstituteFor reconstitution add 100 µl of sterile water.
-
Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applicationswestern blot (WB) Immunohistochemistry (IHC)
-
Connected productsAS03 031S | mPGES-1 glutathione dependent prostaglandin E synthase protein | positive controlSecondary antibodies
-
Recommended dilutions for use1:10 000 on human and rat samples (WB) 1:6 000 on mice samples and in sheep seminal vesicles (IHC)
-
Molecular weight expected аpparent17 kDa
-
Verified reactivityhuman, mouse, rat, sheep
-
Possible reactivitydog, hamster, horse
-
No reactivityno confirmed exceptions from predicted reactivity known in the moment
-
Supplementary informationantibody is only recognizing mPGES1 not mPGES2 or cPGES
-
ReferencesTuure et al. (2014). Aurothiomalate inhibits the expression of mPGES-1 in primary human chondrocytes. Scand J Rheumatol. 2014 Oct 14:1-6. Olajide et al. (2014). Picralima nitida seeds suppress PGE2 production by interfering with multiple signalling pathways in IL-1β-stimulated SK-N-SH neuronal cells. J Ethnopharmacol. 2014 Jan 31. pii: S0378-8741(14)00074-9. doi: 10.1016/j.jep.2014.01.027.
-
Scientific contextmPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.
-
Notesin our hands this antibody works with fresh frozen sections not in paraffin embeded material in IHCmPGES-1 antibody was characterized using spleen tissue from LPS-induced mPGES-1 knock-out and wild type mice. In spleen tissue from wt mice we observed strong positive staining in macrophage-like cells, while there was no staining for mPGES-1 in ko mice.
-
Protein numberO14684
-
TAIR numberRefer to NCBI
-
Gene target
-
Gene symbolIGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1, PIRC1
-
Short namemPGES, PGES-1 glutathione dependent prostaglandin E synthase
-
Alternative namemPGES, PGES-1 GSH dependent prostaglandin E synthase
-
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1/206 family
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-undetermined (NNN) 8-1
-
Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
-
GenBank acession
-
Locus
-
Discovery year2014-06-20
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-undetermined (NNN) 7-1
-
Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
-
GenBank acession
-
Locus
-
Discovery year2014-06-20
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
-
Synonyms gene
-
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Immunoglobulin kappa (IGK) orphons
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 101-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR101 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1289-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2008-11-03
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1289 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 16-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR15/16 family
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 1
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters