Mouse Anti-Biotin Monoclonal antibody

  • Catalog number
    GEN462194
  • Price
    Please ask
  • Size
    100ug
  • Also known as
    Biotin
  • Other names
    N/A
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    Biotin
  • Gene name synonims
    N/A
  • Other gene names
    N/A
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG1, k
  • Clone
    SPM375
  • Host organism
    Mouse (Mus musculus)
  • Species reactivity
    All species; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    This antibody recognizes free Biotin or bound-form biotin, including protein-Biotin, DNA/RNA-Biotin.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    The mouse IgG1 is purified by Protein A-Affinity Chromatography according to Isotyping
  • Form Appearance
    This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
  • Concentration
    Size: 100 ug/200 ul
  • Storage and shipping
    The antibodies are stable for 12 months from date of receipt when stored the antibody should be stored at -20 degrees Celsius. to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
  • Tested applications
    ELISA, Western Blot, Immunohistochemistry
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
  • Short name
    Mouse Anti- Monoclonal antibody
  • Technique
    Antibody, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Label
    Biotin
  • Species
    Mouse, Mouses
  • Alternative name
    Anti-Mouse anti Biotin Monoclonal antibody
  • Alternative technique
    antibodies, murine
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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