Mouse Anti-Biotin Monoclonal antibody
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Catalog number
GEN462194
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Price
Please ask
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Size
100ug
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Also known as
Biotin
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Other names
N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
Biotin
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1, k
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Clone
SPM375
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Host organism
Mouse (Mus musculus)
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Species reactivity
All species; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
This antibody recognizes free Biotin or bound-form biotin, including protein-Biotin, DNA/RNA-Biotin.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
The mouse IgG1 is purified by Protein A-Affinity Chromatography according to Isotyping
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Form Appearance
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
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Concentration
Size: 100 ug/200 ul
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Storage and shipping
The antibodies are stable for 12 months from date of receipt when stored the antibody should be stored at -20 degrees Celsius. to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Tested applications
ELISA, Western Blot, Immunohistochemistry
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Short name
Mouse Anti- Monoclonal antibody
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Technique
Antibody, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Label
Biotin
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Species
Mouse, Mouses
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Alternative name
Anti-Mouse anti Biotin Monoclonal antibody
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Alternative technique
antibodies, murine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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