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Stock availability
In Stock
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Kit s description
Quantitative colorimetric detection of creatinine in urine samples
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Protein target
Urine Creatinine
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Scientific context
Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP (1). Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist (2). Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine (3). Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is
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Primary research area
Cell Signaling, Cardiovascular System
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Category
Assay Detection Kits
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Other name
N-Carbamimidoyl-N-methylglycine Detection Kit, Methylguanidoacetic acid Detection Kit
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Brand name
StressXpress®
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Detection system
Colorimetric Assay
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Assay format
Direct Quantitative Assay
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Main intended usage
Detection kit used to quantitatively measure creatinine in samples.
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Brief protocol
The Urine Creatinine Detection kit is designed to quantitatively measure creatinine present in urine samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. After a short incubation the intensity of the generated color is detected in a microtiter plate reader capable of measuring 490nm wavelength. The concentration of the creatine in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. The Jaffe reaction used in this kit has been modified to read creatinine levels in urine(7,8).
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Kit contents
Clear Microtitre Plates, Creatinine Standard, StressXpress®Creatinine Reagent, Plate Sealer
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Assay precision
Intra Assay Precision: Three human urine samples were diluted 1:20 with deionized water and run in replicates of 20 in an assay. The mean and precision of the calculated creatinine concentrations were: Sample 1- 1174.3 pg/mL, 6% CV Sample 2- 475.9 pg/mL, 5.6% CV Sample 3- 177.4 pg/mL, 14.7% CV Inter Assay Precision: Three human urine samples were diluted 1:20 with deionized water and run in duplicates in 20 assays run over five days by three operators. The mean and precision of the calculated creatinine concentrations were: Sample 1- 1188.1 pg/mL, 7.2% CV Sample 2- 508.7 pg/mL, 6.3% CV Sample 3- 199.7 pg/mL, 10.9% CV
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Assay cross reactivity
Human, Monkey, Dog, Rat
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Samples to be used with this kit
Urine
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Maximum samples to be used with this kit
88 samples in duplicate
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Assay duration
See product datasheet or feel free to contact us
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Kit s sensitivity
0,019 mg/dl
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Assay detection limit
0,3125- 20 mg/dl
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice
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Bibliography
1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.
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Release date
17-Sep-2014
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PubMed number
Refer to PubMed
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Tested applications
No data available / currently testing
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Tested reactivity
No data available / currently testing
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Resources available upon request
Kit Booklet, MSDS
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Product image link
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Product image legend
Typical Standard Curve of Urine Creatinine Detection Kit StressXpress® - SKT-200. Assay Type: Quantitative. Detection Method: Colorimetric Assay. Assay Range: 0.3125- 20 mg/dl | Conversion of creatine and p-creatine to creatinine. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from p-creatine. | Linearity was determined by taking two diluted 1:20 human urine samples, one with a low diluted creatinine level of 0.38 mg/dL and one with a higher diluted level of 9.33 mg/dL and mixing them in the ratios given below. The measured concentrations were compared to the expected values. Graph of the Typical Standard Curve for the Urine Creatinine Detection Kit StressXpress - SKT-200 | Chemical Equation of the Creatine Conversion to Creatinin for the Urine Creatinine Detection Kit StressXpress - SKT-200 | Graph of the Urine Spike Assay for the Urine Creatinine Detection Kit StressXpress - SKT-200
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Warnings
Non-hazardous
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Country of production
Canada
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Total weight kg
2
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Net weight g
300
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Description
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
