Mouse anti Human IgG2 (Fab subclass specific), conjugated with Biotin, Secondary Antibodies
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Catalog number
MAHu-IgG2-Bio
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Price
Please ask
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Size
0,2mg
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Host Source
Mouse
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Clone Name
NI 6014 (HP 6014)
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Product Category
Immunology
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Species Reactivity
Human
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Application
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
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Immunogen
Highly purified monoclonal IgG2 isolated from pooled human serum.
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Purification Method
NA
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Shipping Conditions
NA
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Storage conditions
Mouse anti Human IgG2 (Fab subclass specific), conjugated with Biotin, Secondary Antibodies philized product is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 C, not refrozen, and preferably used the same day. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days
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Datasheet link
NA
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Short name
Mouse anti IgG2 (Fab subclass specific), conjugated with , Secondary Antibodies
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Technique
anti human, Mouse, anti, Fab, antibody to, anti human antibodies, antibody Conjugates, fabs, mouses
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Host
mouse
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Isotype
IgG1, Fab fragment
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Label
Biotin
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Species
Human, Humans, Mouses
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Alternative name
Mouse antibody to H. sapiens IgG2 (Fab subclass specific), coupled including biotinilated, secondary detector Antibody Antibodies
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Alternative technique
murine, antibodies, seconairy
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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