Anti-BRF1 (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-6358R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideBRF1
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SpecificityThis antibody reacts specifically with BRF1
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsNucleus
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Antigen SourceKLH conjugated synthetic peptide derived from human BRF1/RNA polymerase IIIB 90 kDa subunit
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Gene ID2972
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Swiss ProtQ92994
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationGeneral activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsBRF; CFDS; hBRF; BRF-1; GTF3B; TAF3C; TAF3B2; TF3B90; TAFIII90; TFIIIB90; HEL-S-76p; Transcription factor IIIB 90 kDa subunit; hTFIIIB90; B-related factor 1; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2; BRF1
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Also known asBRF1 Polyclonal Antibody
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Other nameAnti-BRF1 Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolBRF1, ZFP36L1
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Short nameAnti-BRF1
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameBRF1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameBRF1 RNA polymerase III transcription initiation factor subunit
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Synonyms gene
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Synonyms gene name
- TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3B subunit 2
- BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)
- BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- General transcription factor IIIB complex subunits
- General transcription factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameZFP36 ring finger protein like 1
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Synonyms gene
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Synonyms gene name
- zinc finger protein, C3H type, 36-like 1
- zinc finger protein 36, C3H type-like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-05-09
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Entrez gene record
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Pubmed identfication
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Classification
- Zinc fingers CCCH-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data