Mouse Anti-Rat Kv3.4 Monoclonal IgG1, Clone S72-16
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Catalog numberSMC-335D-APC
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PricePlease ask
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Size100 µg
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CloneS72-16
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ImmunogenSynthetic peptide amino acids 175-192 of rat Kv3.4
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Antibody s full descriptionMouse Anti-Rat Kv3.4 Monoclonal IgG1 Antibody, Clone: S72-16: APC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameRaw3 Antibody, Voltage-gated potassium channel subunit Kv3.4 Antibody, Potassium voltage-gated channel subfamily C member 4 Antibody
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Raised inMouse
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Antibody s targetKv3.4
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Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Voltage-Gated Potassium Channels, Neurodegeneration, Alzheimer's Disease
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~70kDa (100kDa in brain due to glycosylation).
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-335 was sufficient for detection of Kv3.4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextKv3.4, a member of the Shaw subfamily, may play important roles in maintaining normal function of the corneal epithelium (1). Kv3.4 is also over-expressed in the early stages of Alzheimer’s disease, and therefore represents a novel therapeutic target for this disease (2).
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Bibliography1. Wang L., Fyffe R.E.W., and Lu L. (2004) Investigative Ophthalmology and Visual Science. 45: 1796-1803. 2. Angulo E., et al. (2004) J Neurochem. 91(3): 547-557.
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Released date31-Mar-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001116248.1
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Gene number684516
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Protein numberQ63734
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (SMC-335). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (SMC-335) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive, stains nucleoli . Western Blot analysis of Rat brain membrane lysate showing detection of Kv3.4 Potassium Channel protein using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (SMC-335). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (SMC-335) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (SMC-335). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (SMC-335) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hints of filaggrin-like staining. Mouse Anti-Kv3.4 Potassium Channel Antibody [S72-16] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-335) Mouse Anti-Kv3.4 Potassium Channel Antibody [S72-16] used in Western Blot (WB) on Rat brain membrane lysate (SMC-335) Mouse Anti-Kv3.4 Potassium Channel Antibody [S72-16] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-335)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolESB3, PIRC83, PIRC88, IFNR, PIRC86, PIRC84
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Short nameMouse Anti- Kv3 4 Monoclonal IgG1, Clone S72-16
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelAPC
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat Kv3.4 monoclonal IgG1, clonality S72-16
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Alternative techniquemurine, antibodies
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Clone nameS72-16
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Gene info
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Identity
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Gene
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Long gene nameesterase B3
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Locus
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Discovery year2001-06-22
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 83
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 88
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameinterferon production regulator
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 86
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 84
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data