Mouse Anti-Rat Kv3.4 Monoclonal IgG1, Clone S72-16

  • Catalog number
    SMC-335D-APC
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S72-16
  • Immunogen
    Synthetic peptide amino acids 175-192 of rat Kv3.4
  • Antibody s full description
    Mouse Anti-Rat Kv3.4 Monoclonal IgG1 Antibody, Clone: S72-16: APC
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Raw3 Antibody, Voltage-gated potassium channel subunit Kv3.4 Antibody, Potassium voltage-gated channel subfamily C member 4 Antibody
  • Raised in
    Mouse
  • Antibody s target
    Kv3.4
  • Primary research fields
    Neuroscience, Ion Channels, Potassium Channels, Voltage-Gated Potassium Channels, Neurodegeneration, Alzheimer's Disease
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP, AM
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~70kDa (100kDa in brain due to glycosylation).
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-335 was sufficient for detection of Kv3.4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Kv3.4, a member of the Shaw subfamily, may play important roles in maintaining normal function of the corneal epithelium (1). Kv3.4 is also over-expressed in the early stages of Alzheimer’s disease, and therefore represents a novel therapeutic target for this disease (2).
  • Bibliography
    1. Wang L., Fyffe R.E.W., and Lu L. (2004) Investigative Ophthalmology and Visual Science. 45: 1796-1803. 2. Angulo E., et al. (2004) J Neurochem. 91(3): 547-557.
  • Released date
    31-Mar-2010
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001116248.1
  • Gene number
    684516
  • Protein number
    Q63734
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (SMC-335). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (SMC-335) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive, stains nucleoli . Western Blot analysis of Rat brain membrane lysate showing detection of Kv3.4 Potassium Channel protein using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (SMC-335). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (SMC-335) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (SMC-335). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (SMC-335) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hints of filaggrin-like staining. Mouse Anti-Kv3.4 Potassium Channel Antibody [S72-16] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-335) Mouse Anti-Kv3.4 Potassium Channel Antibody [S72-16] used in Western Blot (WB) on Rat brain membrane lysate (SMC-335) Mouse Anti-Kv3.4 Potassium Channel Antibody [S72-16] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-335)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • Gene target
    Kv3   Monoclonal   IgG1   S72-16  
  • Gene symbol
    ESB3, PIRC83, PIRC88, IFNR, PIRC86, PIRC84
  • Short name
    Mouse Anti- Kv3 4 Monoclonal IgG1, Clone S72-16
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    APC
  • Species
    Rat, Mouses, Rats
  • Alternative name
    Mouse Antibody toRat Kv3.4 monoclonal IgG1, clonality S72-16
  • Alternative technique
    murine, antibodies
  • Clone name
    S72-16
Gene info
  • Identity
  • Gene
  • Long gene name
    esterase B3
  • Locus
    16
  • Discovery year
    2001-06-22
  • Pubmed identfication
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 83
  • Locus
    16
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 88
  • Locus
    16
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    interferon production regulator
  • Locus
    16
  • Discovery year
    1986-01-01
  • Entrez gene record
  • Pubmed identfication
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 86
  • Locus
    16
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 84
  • Locus
    16
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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