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Category
Primary Antibodies
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Long description
Human cyclin E was originally identified by complementation of a triple cln deletion in Saccharomyces cerevisiae. Following the identification of human cyclin E protein, researchers found that breast cancers and some other solid tumors exhibited both quantitative and qualitative alterations in cyclin E protein production. In particular, the alterations in cyclin E expression in breast cancer correlated with increasing stage and grade of the tumor. These results suggested that cyclin E might be useful as a prognostic marker. More recently, Geng et al generated a mouse strain in which the coding sequences for cyclin D1 was deleted and replaced with those of human cyclin E. Replacement of cyclin D1 with cyclin E rescued all phenotypic manifestations seen in the cyclin D1 deficient mice and restored normal development to cyclin D1-dependent tissues. Based on the results of these studies, it appears that cyclin E is the major downstream target of cyclin D1._x000B__x000B_In addition to functioning downstream of cyclin D1, recent studies with Xenopus egg extracts demonstrated that cyclin E localizes to the centrosome. In these studies CDK2-cyclin E activity was shown to be required for centrosome duplication during S phase. Taken together, these data suggest a mechanism that coordinates centrosome reproduction with cycles of DNA synthesis and mitosis.
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Antibody come from
Recombinant full-length human cyclin E protein
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
168
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Antigen antibody binding interaction
Mouse anti cyclin E Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunoprecipitation
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Antibody s suited for
Antibody detects human cyclin E protein by Western blot. Detects a 50 kDa band. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Koff, A., et al. 'Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. Cell 1991, 66, 1217-1228._x000B__x000B_2. Keyomarsi, K., et al. 'Cyclin E, a potential prognostic marker for breast cancer. Cancer Res. 1994, 54, 380-385. _x000B__x000B_3. Geng, Y., et al. 'Rescue of cyclin D1 deficiency by knockin cyclin E. Cell 1999, 97, 767-777. _x000B__x000B_4. Hinchcliffe, E.H., et al. 'Requirement of Cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus egg extracts. Science 1999, 283, 851-854.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
