XRCC5 Polyclonal Antibody
-
Catalog number
CAC07141
-
Price
Please ask
-
Size
50 μg
-
-
Other name
X-ray repair cross-complementing protein 5 (EC:3.6.4.-),XRCC5,G22P2,86 kDa subunit of Ku antigen,ATP-dependent DNA helicase 2 subunit 2,ATP-dependent DNA helicase II 80 kDa subunit,CTC box-binding factor 85 kDa subunit,CTC85,CTCBF,DNA repair protein XRCC5,Ku80,Ku86,Lupus Ku autoantigen protein p86,Nuclear factor IV,Thyroid-lupus autoantigen,TLAA,X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
-
Immunogen
Recombinant human X-ray repair cross-complementing protein 5 protein (251-455AA)
-
Verified reactivity
Human
-
Verified applications
ELISA,WB,IHC. Recommended dilution:WB:1:500-2000,IHC:1:20-1:200
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Protein number
P13010
-
Gene number
Please refer to GenBank
-
NCBI number
Please refer to NCBI
-
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
-
Storage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
-
Use before
1 year
-
Shipping requirements
Blue ice
-
Estimated production time
3-7 business days
-
Supplementary information
Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
-
Notes
For research use only. Not for diagnostic procedures.
-
-
Properties
If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Gene symbol
XRCC5
-
Short name
XRCC5 Polyclonal Antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Label
Unconjugated
-
Alternative name
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), KARP-1 and KARP1 and KU80 and Ku86 and KUB2 and NFIV, XRCC5 and IDBG-80616 and ENSG00000079246 and 7520, hydrolase activity, nuclei, Xrcc5 and IDBG-165686 and ENSMUSG00000026187 and 22596, BT.88138 and IDBG-643715 and ENSBTAG00000010426 and 531945
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
